Draft Genome Sequence of Escherichia coli Strain SEC470, Isolated from a Piglet Experiencing Diarrhea.

Escherichia coli strain SEC470 is a diarrhea-causing strain, isolated from a piglet experiencing serious diarrhea in Jingxi Province, China. Here, we present the draft genome of this strain, which provides the genetic basis for exploring the mechanism of enterotoxigenic E. coli infections.

GENOME ANNOUNCEMENT

Enterotoxigenic Escherichia coli (ETEC) is the most common cause for travelers’ disease and a major cause of infectious diarrhea for infants and newborn animals in developing countries (1). Here, we report the newly sequenced E. coli strain SEC470, isolated from a piglet experiencing serious diarrhea in Jingxi Province, China (2, 3).

E. coli SEC 470 is an O4 serotype strain of ETEC that causes diarrhea in piglets (4). E. coli SEC 470 genomic DNA was extracted using a QIAamp DNA mini kit (Qiagen) according to the manufacturer’s protocol. About 3 ng of genomic DNA was used to generate a library using the Nextera XT kit (Illumina). Micro-Seq Enterprises sequenced the libraries on a MiSeq sequencing system (Illumina). The sequencing generated approximately 6 million read pairs, constituting about 100-fold coverage of the genome. A de novo assembly (SOAPdenovo version 2.04) was applied to assemble the raw FASTQ sequences into a total length of 5.15 Mb (about100-fold coverage) (5). Annotation was done using the Rapid Annotations Using Subsystems Technology (RAST) server (6).

The genome sequence of E. coli SEC 470 comprises 5,153,435 bp, and the annotation revealed 5,192 open reading frames. The availability of the E. coli SEC 470 draft genome sequence provides opportunities for exploring the mechanism of ETEC infections.

Nucleotide sequence accession number.

This whole-genome shotgun project has been deposited at GenBank under the accession number CP013962. The strain is available from Lei Yu (China Animal Disease Control Center, Beijing, China).