Bakiah Shaharuddin1,2, Charles Osei-Bempong1, Sajjad Ahmad3,4, Paul Rooney5, Simi Ali6, Rachel Oldershaw7, Annette Meeson1. 1. Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle Upon-Tyne, NE1 3BZ, UK. 2. Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200 Pulau Pinang, Malaysia. 3. St Paul's Eye Unit, Royal Liverpool University Hospital, Prescot Street, Liverpool, L7 8XP, UK. 4. Department of Eye & Vision Sciences, Institute of Ageing & Chronic Disease, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, L69 3GA, UK. 5. Tissue Development Laboratory, NHS Blood & Transplant, Estuary Banks, Liverpool, L24 8RB, UK. 6. Institute of Cellular Medicine, Newcastle University, Newcastle Upon-Tyne, NE2 4HH, UK. 7. Department of Musculoskeletal Biology Group I, Institute of Ageing & Chronic Disease, Faculty of Health & Life Sciences, University of Liverpool, Leahurst Campus, Chester High Road, Neston, Cheshire, CH64 7TE, UK.
Abstract
AIM: To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. MATERIALS & METHODS: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization. RESULTS: LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers. CONCLUSION: Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.
AIM: To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. MATERIALS & METHODS: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization. RESULTS: LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers. CONCLUSION: Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.
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