| Literature DB >> 26962997 |
Hye Jeong Lee1,2, Na Young Choi1,2, Seung-Won Lee1,2, Kisung Ko3, Tae Sook Hwang4, Dong Wook Han1,2, Jisun Lim5, Hans R Schöler6,7, Kinarm Ko1,2,8.
Abstract
Spermatogonial stem cells (SSCs), which are unipotent stem cells in the testes that give rise to sperm, can be converted into germline-derived pluripotent stem (gPS) by self-induction. The androgenetic imprinting pattern of SSCs is maintained even after their reprogramming into gPS cells. In this study, we used an in vitro neural differentiation model to investigate whether the imprinting patterns are maintained or altered during differentiation. The androgenetic patterns of H19, Snrpn, and Mest were maintained even after differentiation of gPS cells into NSCs (gPS-NSCs), whereas the fully unmethylated status of Ndn in SSCs was altered to somatic patterns in gPS cells and gPS-NSCs. Thus, our study demonstrates epigenetic alteration of genomic imprinting during the induction of pluripotency in SSCs and neural differentiation, suggesting that gPS-NSCs can be a useful model to study the roles of imprinted genes in brain development and human neurodevelopmental disorders.Entities:
Keywords: Androgenetic imprinting; germline-derived pluripotent stem cells; in vitro model; neural stem cells; spermatogonial stem cells
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Year: 2016 PMID： 26962997 PMCID： PMC4854545 DOI： 10.1080/15592294.2016.1146852
Source DB: PubMed Journal: Epigenetics ISSN： 1559-2294 Impact factor: 4.528