Bamidele Tolulope Odumosu1, Bolanle A Adeniyi2, Ram Chandra3. 1. Department of Microbiology University of Lagos, Akoka Lagos; Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria; Environmental Microbiology Section, Indian Institute of Toxicology Research, Lucknow, India. 2. Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria. 3. Environmental Microbiology Section, Indian Institute of Toxicology Research, Lucknow, India.
Abstract
BACKGROUND: Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa. OBEJECTIVES: We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria. METHODS: A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs. RESULTS AND CONCLUSION: Two types of AME genes [aac (6') - I and ant (2″) - I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6') - I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6″) - II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.
BACKGROUND: Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa. OBEJECTIVES: We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria. METHODS: A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs. RESULTS AND CONCLUSION: Two types of AME genes [aac (6') - I and ant (2″) - I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6') - I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6″) - II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.
Authors: G H Miller; F J Sabatelli; R S Hare; Y Glupczynski; P Mackey; D Shlaes; K Shimizu; K J Shaw Journal: Clin Infect Dis Date: 1997-01 Impact factor: 9.079
Authors: Min Song; Min Tang; Yinghuan Ding; Zecai Wu; Chengyu Xiang; Kui Yang; Zhang Zhang; Baolin Li; Zhenghua Deng; Jinbo Liu Journal: Environ Sci Pollut Res Int Date: 2017-12-16 Impact factor: 4.223