Zhu-Gen Cao1, Xiao-Bing Qin2, Fang-Fang Liu3, Lei-Lei Zhou4. 1. Department of Oncology, Si'hong County People's Hospital, Si'hong, Jiangsu, 223900, P.R. China. 2. Department of Oncology, The First People's Hospital of Xuzhou, Xu'zhou, Jiangsu, 221002, P.R. China. 3. Department of General Surgery, Huai'an Second People's Hospital, Huai'an, Jiangsu223002, P.R. China. 4. Department of Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R.China.
Abstract
BACKGROUND: The pathogenesis of breast cancer remains unclear. AIMS: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer. METHODS: Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell. RESULTS: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration. CONCLUSION: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.
BACKGROUND: The pathogenesis of breast cancer remains unclear. AIMS: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer. METHODS:Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell. RESULTS: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration. CONCLUSION: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.
Entities:
Keywords:
T cell; breast cancer; interleukin-10 (IL-10); pathogenesis
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