Fang Guo1, Xiyun Ren2, Yingzi Dong1, Xiaomeng Hu1, Dan Xu3, Haibo Zhou1, Fanyu Meng4, Wenjing Tian5, Yashuang Zhao6. 1. Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China. 2. Teaching Center for Preventive Medicine Experiments, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China. 3. Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, PR China. 4. Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin, Heilongjiang Province, PR China. 5. Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China. Electronic address: twj8267@sina.com. 6. Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China. Electronic address: zhao_yashuang@263.net.
Abstract
BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the PPAR nuclear hormone receptor superfamily, which plays a crucial role in carcinogenesis. Wnt/β-Catenin signaling pathway has been well certified to contribute to the progression of gastric malignancies. β-Catenin mediates transcriptional regulation by forming a complex with LEF/TCF transcription factors, resulting in activation of downstream target genes such as TERT, ENAH. In this study, we aimed at detecting the effect of PPARγ on TERT, ENAH and explaining the further mechanisms of PPARγ on tumor suppression. METHODS: The pEGFP-N1-PPARγ recombinant plasmid has already been constructed by researchers in our laboratory. We stably transfected it into three gastric cancer (GC) cell lines (MKN-28, SGC-7901 and BGC-823). CCK-8 and transwell assay were employed to analyze the capability of cell proliferation and metastasis. The mRNA and protein levels were evaluated by real-time PCR and western blot analysis. RESULTS: After transfected with PPARγ overexpression plasmid, the ability of cell proliferation and migration declined significantly (p<0.05). The expression of PPARγ increased (p<0.05) and β-Catenin was inhibited obviously (p<0.05) in the group of pEGFP-N1-PPARγ plasmid transfection. Meanwhile, the mRNA or protein levels of TERT and ENAH were suppressed (p<0.05) in pEGFP-N1-PPARγ plasmid transfection group compared with control groups. CONCLUSION: PPARγ might inhibit the proliferation and migration of GC cell lines through suppressing the expression of TERT and ENAH. PPARγ played an important role as a physiological regulator and might be a target for the treatment of GC.
BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the PPAR nuclear hormone receptor superfamily, which plays a crucial role in carcinogenesis. Wnt/β-Catenin signaling pathway has been well certified to contribute to the progression of gastric malignancies. β-Catenin mediates transcriptional regulation by forming a complex with LEF/TCF transcription factors, resulting in activation of downstream target genes such as TERT, ENAH. In this study, we aimed at detecting the effect of PPARγ on TERT, ENAH and explaining the further mechanisms of PPARγ on tumor suppression. METHODS: The pEGFP-N1-PPARγ recombinant plasmid has already been constructed by researchers in our laboratory. We stably transfected it into three gastric cancer (GC) cell lines (MKN-28, SGC-7901 and BGC-823). CCK-8 and transwell assay were employed to analyze the capability of cell proliferation and metastasis. The mRNA and protein levels were evaluated by real-time PCR and western blot analysis. RESULTS: After transfected with PPARγ overexpression plasmid, the ability of cell proliferation and migration declined significantly (p<0.05). The expression of PPARγ increased (p<0.05) and β-Catenin was inhibited obviously (p<0.05) in the group of pEGFP-N1-PPARγ plasmid transfection. Meanwhile, the mRNA or protein levels of TERT and ENAH were suppressed (p<0.05) in pEGFP-N1-PPARγ plasmid transfection group compared with control groups. CONCLUSION: PPARγ might inhibit the proliferation and migration of GC cell lines through suppressing the expression of TERT and ENAH. PPARγ played an important role as a physiological regulator and might be a target for the treatment of GC.