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Literature DB >> 2695395

Cloning and sequence analysis of the ntrA (rpoN) gene of Pseudomonas putida.

S Inouye¹, M Yamada, A Nakazawa, T Nakazawa.

Abstract

The gene encoding a sigma factor NtrA (RpoN) was cloned from Pseudomonas putida by cross-hybridization with a probe containing a part of the corresponding Escherichia coli gene. The cloned gene complemented an ntrA mutation of E. coli in activation of xyl genes on the TOL plasmid. The predicted amino acid (aa) sequence of P. putida NtrA (497 aa; Mr 56,215) is highly homologous to NtrA proteins from Azotobacter vinelandii (81.7%), Klebsiella pneumoniae (52.6%), and Rhizobium meliloti (36.1%). There are two other open reading frames (ORF1, ORF3) upstream and downstream from the ntrA gene, respectively. These ntrA-flanking ORFs are also highly homologous to the corresponding ORFs of K. pneumoniae, A. vinelandii, and R. meliloti.

Entities: Gene Species

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Year: 1989 PMID： 2695395 DOI： 10.1016/0378-1119(89)90474-5

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

15 in total

7. Analysis of an upstream regulatory sequence required for activation of the regulatory gene xylS in xylene metabolism directed by the TOL plasmid of Pseudomonas putida.

Authors: M Gomada; S Inouye; H Imaishi; A Nakazawa; T Nakazawa
Journal: Mol Gen Genet Date: 1992-06

8. The functional importance of structural differences between the mannitol-specific IIAmannitol and the regulatory IIAnitrogen.

Authors: R L van Montfort; B W Dijkstra
Journal: Protein Sci Date: 1998-10 Impact factor: 6.725

9. RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.

Authors: S Ehrt; L N Ornston; W Hillen
Journal: J Bacteriol Date: 1994-06 Impact factor: 3.490

10. Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.

Authors: M A Farinha; S L Ronald; A M Kropinski; W Paranchych
Journal: Infect Immun Date: 1993-04 Impact factor: 3.441

Cloning and sequence analysis of the ntrA (rpoN) gene of Pseudomonas putida.

1. Isolation and characterization of the nifUSVW-rpoN gene cluster from Rhodobacter sphaeroides.

2. A proposed link between nitrogen and carbon metabolism involving protein phosphorylation in bacteria.

3. Virulence of the phytopathogen Pseudomonas syringae pv. maculicola is rpoN dependent.

4. Physical map location of the rpoN gene of Escherichia coli.

5. Location of the rpoN gene on the physical map of Escherichia coli.

6. A variety of DNA-binding and multimeric proteins contain the histone fold motif.

7. Analysis of an upstream regulatory sequence required for activation of the regulatory gene xylS in xylene metabolism directed by the TOL plasmid of Pseudomonas putida.

8. The functional importance of structural differences between the mannitol-specific IIAmannitol and the regulatory IIAnitrogen.

9. RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.

10. Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.