Edvin Prifti1, Gabriella Di Lascio2, Guy Harmelin3, Daniele Bani4, Vittorio Briganti5, Altin Veshti6, Massimo Bonacchi3. 1. Division of Cardiac Surgery, University Hospital Center of Tirana, Albania. Electronic address: edvinprifti@hotmail.com. 2. Anesthesiology and Intensive Care Section, Department of Health Sciences, University of Florence, Florence, Italy. 3. Cardiac Surgery, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. 4. Research Unit of Histology & Embryology, Departments of Clinical & Experimental Medicine, University of Florence, Florence, Italy. 5. Unit of Nuclear Medicine, Careggi Hospital, Florence, Italy. 6. Division of Cardiac Surgery, University Hospital Center of Tirana, Albania.
Abstract
OBJECTIVES: The aim was to create a model of myocardial infarction with a borderline myocardial impairment which would enable evaluation of the retrograde cellular cardiomyoplasty through the venous coronary sinus in a large animal model. MATERIALS AND METHODS: Fifteen (study group) and 10 juvenile farm pigs (control group) underwent distal left anterior descending artery ligation. One month later the study group animals underwent sternotomy and a murine myoblastic line C2-C12 was injected at a constant pressure of 30mmHg, into the coronary sinus. Thirty days later all animals that survived from both groups underwent transthoracic echocardiography and 99Tc scintigraphy and were later euthanized and specimens were taken for microscopic evaluation. RESULTS: Cardiac output decreased significantly after ligation (p<0.001) and increased significantly after cardiomyoplasty (p<0.001). In all animals, the surgical induction of myocardial infarction caused a marked decline in the echocardiographic values of cardiac function; however, the cardiac function and dimensions were significantly improved in the study group after cardiomyoplasty versus the control group. All animals undergoing cardiomyoplasty demonstrated a significant reduction of the perfusion deficit in the left anterior descending artery territory, instead such data remained unchanged in the control group. The histological examination demonstrated the engrafted myoblasts could be distinguished from the activated fibroblasts in the scar tissue because they never showed any signs of collagen secretion and fiber buildup. CONCLUSIONS: In conclusion, the venous retrograde delivery route through the coronary sinus is safe and effective, providing a significant improvement in function and viability.
OBJECTIVES: The aim was to create a model of myocardial infarction with a borderline myocardial impairment which would enable evaluation of the retrograde cellular cardiomyoplasty through the venous coronary sinus in a large animal model. MATERIALS AND METHODS: Fifteen (study group) and 10 juvenile farm pigs (control group) underwent distal left anterior descending artery ligation. One month later the study group animals underwent sternotomy and a murine myoblastic line C2-C12 was injected at a constant pressure of 30mmHg, into the coronary sinus. Thirty days later all animals that survived from both groups underwent transthoracic echocardiography and 99Tc scintigraphy and were later euthanized and specimens were taken for microscopic evaluation. RESULTS: Cardiac output decreased significantly after ligation (p<0.001) and increased significantly after cardiomyoplasty (p<0.001). In all animals, the surgical induction of myocardial infarction caused a marked decline in the echocardiographic values of cardiac function; however, the cardiac function and dimensions were significantly improved in the study group after cardiomyoplasty versus the control group. All animals undergoing cardiomyoplasty demonstrated a significant reduction of the perfusion deficit in the left anterior descending artery territory, instead such data remained unchanged in the control group. The histological examination demonstrated the engrafted myoblasts could be distinguished from the activated fibroblasts in the scar tissue because they never showed any signs of collagen secretion and fiber buildup. CONCLUSIONS: In conclusion, the venous retrograde delivery route through the coronary sinus is safe and effective, providing a significant improvement in function and viability.
Authors: Wouter A Gathier; Dirk Jan van Ginkel; Mira van der Naald; Frebus J van Slochteren; Pieter A Doevendans; Steven A J Chamuleau Journal: J Cardiovasc Transl Res Date: 2018-02-01 Impact factor: 4.132
Authors: Emma J Agnew; Nivedhitha Velayutham; Gabriela Matos Ortiz; Christina M Alfieri; Luis Hortells; Victoria Moore; Kyle W Riggs; R Scott Baker; Aaron M Gibson; Sithara Raju Ponny; Tarek Alsaied; Farhan Zafar; Katherine E Yutzey Journal: J Cardiovasc Dev Dis Date: 2019-12-18
Authors: Wouter A Gathier; Mira van der Naald; Bas R van Klarenbosch; Anton E Tuinenburg; John Lm Bemelmans; Klaus Neef; Joost Pg Sluijter; Frebus J van Slochteren; Pieter A Doevendans; Steven Aj Chamuleau Journal: BMJ Open Sci Date: 2019-01-07