| Literature DB >> 26953100 |
Manu P Gangola1, Sarita Jaiswal1, Udhaya Kannan1, Pooran M Gaur2, Monica Båga1, Ravindra N Chibbar3.
Abstract
To understand raffinose family oligosaccharides (RFO) metabolism in chickpea (Cicer arietinum L.) seeds, RFO accumulation and corresponding biosynthetic enzymes activities were determined during seed development of chickpea genotypes with contrasting RFO concentrations. RFO concentration in mature seeds was found as a facilitator rather than a regulating step of seed germination. In mature seeds, raffinose concentrations ranged from 0.38 to 0.68 and 0.75 to 0.99 g/100 g, whereas stachyose concentrations varied from 0.79 to 1.26 and 1.70 to 1.87 g/100 g indicating significant differences between low and high RFO genotypes, respectively. Chickpea genotypes with high RFO concentration accumulated higher concentrations of myo-inositol and sucrose during early seed developmental stages suggesting that initial substrate concentrations may influence RFO concentration in mature seeds. High RFO genotypes showed about two to three-fold higher activity for all RFO biosynthetic enzymes compared to those with low RFO concentrations. RFO biosynthetic enzymes activities correspond with accumulation of individual RFO during seed development.Entities:
Keywords: Chickpea; Cicer arietinum L.; Fabaceae; Galactinol synthase; Raffinose family oligosaccharides (RFO); Seed germination; Seed weight/size
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Year: 2016 PMID： 26953100 DOI： 10.1016/j.phytochem.2016.02.009
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072