Aylin Hatice Yamac1, Sitki Kucukbuzcu1, Mehmet Ozansoy2, Ozlem Gok3, Kursat Oz4, Mehmet Erturk5, Emre Yilmaz5, Burak Ersoy4, Rahmi Zeybek6, Omer Goktekin1, Ulkan Kilic7. 1. Bezmialem Vakif University, Faculty of Medicine, Department of Cardiology, Istanbul, Turkey. 2. Istanbul Medipol University, Faculty of Medicine, Department of Physiology and Regenerative and Restorative Medicine Research Center (REMER), Istanbul, Turkey. 3. Istanbul Medipol University, Vocational School of Health Services, Istanbul, Turkey. 4. Mehmet Akif Ersoy Heart Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey. 5. Mehmet Akif Ersoy Heart Hospital, Department of Cardiology, Istanbul, Turkey. 6. Bezmialem Vakif University, Department of Cardiovascular Surgery, Istanbul, Turkey. 7. Istanbul Medipol University, Faculty of Medicine, Department of Medical Biology and Regenerative and Restorative Medicine Research Center (REMER), Istanbul, Turkey. Electronic address: uckilic@yahoo.com.
Abstract
BACKGROUND: Postoperative atrial fibrillation (POAF) is a potentially life-threatening complication after coronary artery bypass graft (CABG) surgery. The expression of the cardioprotective SIRT1 protein with its antioxidant activity is increased in cardiac tissue of patients suffering from POAF. So far, information is lacking about the relationship between SIRT1 regulating micro RNAs (miRs), SIRT1 protein and the occurrence of POAF. METHODS: A total of 63 patients undergoing CABG were recruited, and biopsies were obtained from the right atrial appendage during cannulation. Postoperative, all patients were rhythm-monitored until discharge and randomized to POAF (n=20) or sinus rhythm (n=43). The expression of the micro RNAs miR-199a and miR-195 was quantified by real-time PCR. SIRT1 protein was detected by western blot analysis. RESULTS: The relative expression of miR-199a in the POAF group was significantly decreased compared to the control group (0.77±0.27 vs. 1.11±0.69, P=.022) Accordingly, SIRT 1 protein was significantly induced in tissue probes of patients with POAF (P<.001). CONCLUSION: Altered expression of the SIRT1 protein regulating miR-199a in human atrial tissue was found to be related to the occurrence of POAF, indicating its usefulness as a biomarker for cardiac surgery management.
BACKGROUND:Postoperative atrial fibrillation (POAF) is a potentially life-threatening complication after coronary artery bypass graft (CABG) surgery. The expression of the cardioprotective SIRT1 protein with its antioxidant activity is increased in cardiac tissue of patients suffering from POAF. So far, information is lacking about the relationship between SIRT1 regulating micro RNAs (miRs), SIRT1 protein and the occurrence of POAF. METHODS: A total of 63 patients undergoing CABG were recruited, and biopsies were obtained from the right atrial appendage during cannulation. Postoperative, all patients were rhythm-monitored until discharge and randomized to POAF (n=20) or sinus rhythm (n=43). The expression of the micro RNAs miR-199a and miR-195 was quantified by real-time PCR. SIRT1 protein was detected by western blot analysis. RESULTS: The relative expression of miR-199a in the POAF group was significantly decreased compared to the control group (0.77±0.27 vs. 1.11±0.69, P=.022) Accordingly, SIRT 1 protein was significantly induced in tissue probes of patients with POAF (P<.001). CONCLUSION: Altered expression of the SIRT1 protein regulating miR-199a in human atrial tissue was found to be related to the occurrence of POAF, indicating its usefulness as a biomarker for cardiac surgery management.
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