Maria Teresa Arias-Moliz1, Ana Morago2, Ronald Ordinola-Zapata3, Carmen María Ferrer-Luque4, Matilde Ruiz-Linares4, Pilar Baca4. 1. Department of Microbiology, School of Dentistry, University of Granada, Spain. Electronic address: mtarias@ugr.es. 2. Department of Microbiology, School of Dentistry, University of Granada, Spain. 3. Department of Endodontics, Bauru Dental School, University of São Paulo, São Paulo, Brazil. 4. Department of Stomatology, School of Dentistry, University of Granada, Spain.
Abstract
INTRODUCTION: The purpose of this study was to determine the influence of dentin powder on the concentration, pH, and antimicrobial activity of sodium hypochlorite (NaOCl) alone and combined with etidronic acid (HEBP). METHODS: Biofilms of Enterococcus faecalis were grown on the surface of dentin blocks for 5 days and then exposed to 1% and 2.5% NaOCl alone or combined with 9% HEBP for 3 minutes in the absence and presence of dentin powder. The biovolumes of the biofilm were measured using confocal microscopy and the live/dead technique. The available chlorine and pH of the solutions were also measured. Nonparametric tests were used to determine statistical differences (P < .05). RESULTS: The presence of dentin powder resulted in a reduction of the free available chlorine and pH in all the irrigating solutions; 1% NaOCl lost its antimicrobial activity completely in the presence of dentin powder. The antimicrobial activity was significantly reduced in the 2.5% NaOCl and 1% NaOCl/HEBP groups, and it was not affected in the 2.5% NaOCl/HEBP group. CONCLUSIONS: The presence of dentin powder significantly decreased the available chlorine and antimicrobial activity of 1% NaOCl, 2.5% NaOCl, and 1% NaOCl/HEBP irrigating solutions. The antimicrobial activity of 2.5% NaOCl/HEBP was not affected by the dentin powder after a 3-minute contact time against E. faecalis biofilms.
INTRODUCTION: The purpose of this study was to determine the influence of dentin powder on the concentration, pH, and antimicrobial activity of sodium hypochlorite (NaOCl) alone and combined with etidronic acid (HEBP). METHODS: Biofilms of Enterococcus faecalis were grown on the surface of dentin blocks for 5 days and then exposed to 1% and 2.5% NaOCl alone or combined with 9% HEBP for 3 minutes in the absence and presence of dentin powder. The biovolumes of the biofilm were measured using confocal microscopy and the live/dead technique. The available chlorine and pH of the solutions were also measured. Nonparametric tests were used to determine statistical differences (P < .05). RESULTS: The presence of dentin powder resulted in a reduction of the free available chlorine and pH in all the irrigating solutions; 1% NaOCl lost its antimicrobial activity completely in the presence of dentin powder. The antimicrobial activity was significantly reduced in the 2.5% NaOCl and 1% NaOCl/HEBP groups, and it was not affected in the 2.5% NaOCl/HEBP group. CONCLUSIONS: The presence of dentin powder significantly decreased the available chlorine and antimicrobial activity of 1% NaOCl, 2.5% NaOCl, and 1% NaOCl/HEBP irrigating solutions. The antimicrobial activity of 2.5% NaOCl/HEBP was not affected by the dentin powder after a 3-minute contact time against E. faecalis biofilms.
Authors: T C Pereira; R J B Dijkstra; X Petridis; W J van der Meer; P K Sharma; F B de Andrade; L W M van der Sluis Journal: Int Endod J Date: 2020-08-27 Impact factor: 5.264
Authors: Mariana Maciel Batista Borges; René J B Dijkstra; Flaviana Bombarda de Andrade; Marco Antonio Hungaro Duarte; Michel Versluis; Lucas W M van der Sluis; Xenos Petridis Journal: Int Endod J Date: 2022-05-06 Impact factor: 5.165