Matthias Napp1, Georg Daeschlein2, Sebastian von Podewils2, Peter Hinz3, Steffen Emmert4, Hermann Haase2, Romy Spitzmueller3, Denis Gümbel3, Richard Kasch5, Michael Jünger2. 1. Clinic and Polyclinic for Trauma Surgery and Rehabilitative Medicine, Ernst Moritz Arndt University, Sauerbruchstraße, 17475, Greifswald, Germany. matthias.napp@uni-greifswald.de. 2. Clinic and Polyclinic of Dermatology, Ernst Moritz Arndt University, Sauerbruchstraße, 17489, Greifswald, Germany. 3. Clinic and Polyclinic for Trauma Surgery and Rehabilitative Medicine, Ernst Moritz Arndt University, Sauerbruchstraße, 17475, Greifswald, Germany. 4. Clinic and Polyclinic of Dermatology and Venerology, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany. 5. Clinic and Polyclinic of Orthopaedics and Orthopaedic Surgery, Ernst Moritz Arndt University, Sauerbruchstraße, 17475, Greifswald, Germany.
Abstract
BACKGROUND: Staphylococcus aureus may be the most important wound pathogen and causative for most of surgical site infections. As many anti-staphylococcal drugs are useless because of resistance, novel antimicrobial strategies are strongly needed and may be provided by cold atmospheric plasma (CP), which is being currently investigated for antiseptic efficacy. METHODS: To test the antimicrobial properties of CP against Staphylococcus aureus, 168 methicillin-susceptible isolates (MSSA) and 50 methicillin-resistant isolates (MRSA) were treated with two technically different plasma sources [an atmospheric pressure plasma jet (APPJ) and a dielectric barrier discharge plasma (DBD)] in vitro. RESULTS: CP treatment allowed a reproducible and significant growth reduction of MRSA and MSSA. However, MRSA was significantly less susceptible to treatment with DBD than was MSSA, while no difference between MRSA and MSSA was found using APPJ. CONCLUSIONS: As the initial physical antiseptic on skin, CP may be suitable for rapid decolonization of microbial pathogens in vivo. Each device must undergo validated efficacy testing prior to clinical application, as device related differences may occur.
BACKGROUND:Staphylococcus aureus may be the most important wound pathogen and causative for most of surgical site infections. As many anti-staphylococcal drugs are useless because of resistance, novel antimicrobial strategies are strongly needed and may be provided by cold atmospheric plasma (CP), which is being currently investigated for antiseptic efficacy. METHODS: To test the antimicrobial properties of CP against Staphylococcus aureus, 168 methicillin-susceptible isolates (MSSA) and 50 methicillin-resistant isolates (MRSA) were treated with two technically different plasma sources [an atmospheric pressure plasma jet (APPJ) and a dielectric barrier discharge plasma (DBD)] in vitro. RESULTS: CP treatment allowed a reproducible and significant growth reduction of MRSA and MSSA. However, MRSA was significantly less susceptible to treatment with DBD than was MSSA, while no difference between MRSA and MSSA was found using APPJ. CONCLUSIONS: As the initial physical antiseptic on skin, CP may be suitable for rapid decolonization of microbial pathogens in vivo. Each device must undergo validated efficacy testing prior to clinical application, as device related differences may occur.
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Authors: Anne Mai-Prochnow; Anthony B Murphy; Keith M McLean; Michael G Kong; Kostya Ken Ostrikov Journal: Int J Antimicrob Agents Date: 2014-03-01 Impact factor: 5.283
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Authors: Tim Maisch; Tetsuji Shimizu; Yang-Fang Li; Julia Heinlin; Sigrid Karrer; Gregor Morfill; Julia L Zimmermann Journal: PLoS One Date: 2012-04-27 Impact factor: 3.240