BACKGROUND: The molecular biological techniques of polymerase chain reaction (PCR) are accurate and rapid diagnostic methods in the epidemiology of Mycobacterium species in humans and animals, especially in developing countries. METHODS: In this study, positive cultures from suspected tuberculous lesions of slaughtered cattle from two abattoirs in north-central Nigeria were subjected to a two-step multiplex PCR technique, based on genus typing and genomic regions of difference (RD). RESULTS: Out of 50 suspected tuberculous lesions cultured, 40 isolates were obtained. Based on genus typing, 32 of the isolates were identified as Mycobacterium tuberculosis complex (MTC), one as non-tuberculous Mycobacterium (NTM) and the remaining seven were unclassified. Using genomic RD multiplex PCR, all the 32 isolates initially identified as MTC were further characterized as M. bovis. CONCLUSION: Our findings show that 80% of positive cultures from suspected tuberculous lesions were identified as M. bovis; hence, re-confirming M. bovis as the main cause of bovine tuberculosis in Nigeria. These results give further credence to the use of PCR-based molecular techniques as excellent complementary epidemiological tools in the tracking of bovine tuberculosis, a zoonotic disease of major public health importance in Nigeria.
BACKGROUND: The molecular biological techniques of polymerase chain reaction (PCR) are accurate and rapid diagnostic methods in the epidemiology of Mycobacterium species in humans and animals, especially in developing countries. METHODS: In this study, positive cultures from suspected tuberculous lesions of slaughtered cattle from two abattoirs in north-central Nigeria were subjected to a two-step multiplex PCR technique, based on genus typing and genomic regions of difference (RD). RESULTS: Out of 50 suspected tuberculous lesions cultured, 40 isolates were obtained. Based on genus typing, 32 of the isolates were identified as Mycobacterium tuberculosis complex (MTC), one as non-tuberculous Mycobacterium (NTM) and the remaining seven were unclassified. Using genomic RD multiplex PCR, all the 32 isolates initially identified as MTC were further characterized as M. bovis. CONCLUSION: Our findings show that 80% of positive cultures from suspected tuberculous lesions were identified as M. bovis; hence, re-confirming M. bovis as the main cause of bovine tuberculosis in Nigeria. These results give further credence to the use of PCR-based molecular techniques as excellent complementary epidemiological tools in the tracking of bovine tuberculosis, a zoonotic disease of major public health importance in Nigeria.