| Literature DB >> 26948583 |
Bingrui Liu1, Kenneth T Kongstad1, Stefanie Wiese2, Anna K Jäger1, Dan Staerk3.
Abstract
Crude chloroform, ethanol and acetone extracts of nineteen seaweed species were screened for their antioxidant and α-glucosidase inhibitory activity. Samples showing more than 60% α-glucosidase inhibitory activity, at a concentration of 1 mg/ml, were furthermore investigated using high-resolution α-glucosidase inhibition profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-HRMS-SPE-NMR). The results showed Ascophyllum nodosum and Fucus vesicolosus to be rich in antioxidants, equaling a Trolox equivalent antioxidant capacity of 135 and 108 mM Troloxmg(-1) extract, respectively. HR-bioassay/HPLC-HRMS-SPE-NMR showed the α-glucosidase inhibitory activity of A. nodosum, F. vesoculosus, Laminaria digitata, Laminaria japonica and Undaria pinnatifida to be caused by phlorotannins as well as fatty acids - with oleic acid, linoleic acid and eicosapentaenoic acid being the most potent with IC50 values of 0.069, 0.075 and 0.10 mM, respectively, and showing a mixed-type inhibition mode.Entities:
Keywords: Antioxidant capacity; Edible seaweed; Functional food; HPLC–HRMS–SPE–NMR; High-resolution inhibition profile; α-Glucosidase inhibition
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Year: 2016 PMID： 26948583 DOI： 10.1016/j.foodchem.2016.02.001
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514