Florence Abravanel1, Hugo Barragué2, Gaëlle Dörr3, Karine Sauné4, Jean-Marie Péron5, Laurent Alric6, Nassim Kamar7, Jacques Izopet4, Eric Champagne2. 1. Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, Centre National de Référence Hépatite E, Institut fédératif de biologie de Purpan, F-31300, Toulouse, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France. Electronic address: abravanel.f@chu-toulouse.fr. 2. Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France. 3. Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Rangueil, Service de Néphrologie, Dialyse et Transplantation multi-organe, F-31049, Toulouse, France. 4. Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, Centre National de Référence Hépatite E, Institut fédératif de biologie de Purpan, F-31300, Toulouse, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France. 5. CHU Toulouse, Hôpital Purpan, Département de Gastroentérologie, F-31300, Toulouse, France. 6. CHU Toulouse, Hôpital Purpan, Service de médecine interne, F-31300, Toulouse, France. 7. Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France; CHU Toulouse, Hôpital Rangueil, Service de Néphrologie, Dialyse et Transplantation multi-organe, F-31049, Toulouse, France.
Abstract
OBJECTIVES: The hepatitis E virus (HEV) causes usually benign and spontaneously resolving acute hepatitis in immunocompetent individuals. In immunocompromised patients with a solid-organ transplant (SOT), chronic infections occur in about 2/3 of cases. We aimed to evaluate the immune cells implicated at the acute phase of HEV infection. METHODS: We studied the activation and memory markers on CD4, CD8, γδ and NK cells in 32 HEV-free control SOT patients and 23 SOT recipients, including 14 who became chronically infected. Samples from 7 immunocompetent individuals with an acute infection and 8 healthy donor samples were included for comparison. RESULTS: In acutely-infected SOT patients, NK and Vδ2 cells, but not other γδ cells, had an increased expression of CD69. Based on CD45RA/CD27 markers, solid-organ recipients infected with HEV contained a larger pool of circulating naive subsets among lymphocyte Tγδ cells. However, these alterations of Vδ2 cells were not associated with HEV clearance. Only the adaptive IFN-γ responses to HEV peptides, determined by ELISpot, were associated with a favorable outcome in immunocompromised patients. CONCLUSIONS: Transplanted patients mobilized their γδ cells at the acute phase of infection. Their precise role in HEV infection will thus deserve further investigations as they could be specifically immunomanipulated.
OBJECTIVES: The hepatitis E virus (HEV) causes usually benign and spontaneously resolving acute hepatitis in immunocompetent individuals. In immunocompromised patients with a solid-organ transplant (SOT), chronic infections occur in about 2/3 of cases. We aimed to evaluate the immune cells implicated at the acute phase of HEV infection. METHODS: We studied the activation and memory markers on CD4, CD8, γδ and NK cells in 32 HEV-free control SOT patients and 23 SOT recipients, including 14 who became chronically infected. Samples from 7 immunocompetent individuals with an acute infection and 8 healthy donor samples were included for comparison. RESULTS: In acutely-infected SOT patients, NK and Vδ2 cells, but not other γδ cells, had an increased expression of CD69. Based on CD45RA/CD27 markers, solid-organ recipients infected with HEV contained a larger pool of circulating naive subsets among lymphocyte Tγδ cells. However, these alterations of Vδ2 cells were not associated with HEV clearance. Only the adaptive IFN-γ responses to HEV peptides, determined by ELISpot, were associated with a favorable outcome in immunocompromised patients. CONCLUSIONS: Transplanted patients mobilized their γδ cells at the acute phase of infection. Their precise role in HEV infection will thus deserve further investigations as they could be specifically immunomanipulated.
Authors: Hugo Barragué; Bertrand Condat; Nicolas Petitdidier; Eric Champagne; Christophe Renou; Jacques Izopet; Florence Abravanel Journal: Medicine (Baltimore) Date: 2017-09 Impact factor: 1.889
Authors: Katie Healy; Anna Pasetto; Michał J Sobkowiak; Chai Fen Soon; Markus Cornberg; Soo Aleman; Margaret Sällberg Chen Journal: Cells Date: 2020-06-16 Impact factor: 6.600