Asghar Abdoli1,2, Hoorieh Soleimanjahi1, Abbas Jamali3, Parvaneh Mehrbod3, Shima Gholami3, Zahra Kianmehr4, Neda Feizi3, Maryam Saleh3, Fariborz Bahrami5, Talat Mokhtari-Azad6, Mohsen Abdoli3, Masoumeh Tavassoti Kheiri7. 1. Departments of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran. 3. Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran. 4. Immunoregulation Research Center, Shahed University, Tehran, Iran. 5. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran. 6. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 7. Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran. mtkheiri@gmail.com.
Abstract
OBJECTIVES: To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. RESULTS: Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK-SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. CONCLUSIONS: MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production.
OBJECTIVES: To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. RESULTS: Yields obtained for influenza virusH1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK-SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. CONCLUSIONS: MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production.