Antonio Arnaiz-Villena1, Jose Palacio-Grüber2, Ester Muñiz2, Diego Rey2, Maria Jose Recio3, Cristina Campos2, Narcisa Martinez-Quiles3, Jose Manuel Martin-Villa3, Jorge Martinez-Laso4. 1. Department of Immunology, University Complutense, School of Medicine, The Madrid Regional Blood Center, Madrid, Spain. Electronic address: arnaizantonio@gmail.com. 2. Department of Immunology, University Complutense, School of Medicine, The Madrid Regional Blood Center, Madrid, Spain. 3. Department of Immunology, University Complutense, School of Medicine, Madrid, Spain. 4. Immunogenetics and Molecular Diagnosis Unit, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
Abstract
BACKGROUND: HLA-DMB proteins are important for intracellular microbial metabolism in order other major histocompatibility complex (MHC) molecules present peptides to lymphocytes. In addition, HLA-DMB alleles have been found linked to diseases in some ethnic groups and HLA-DMB molecules may be important to explain HLA disease association. OBJECTIVE: To detect HLA-DMB alleles profile in Amerindians for the first time and compare them to other populations. This will establish the bases to study HLA-DMB linkage to disease in Amerindians. METHOD: A group of 168 voluntary Amerindians have been typed for HLA-DMB alleles. They have been characterized both, by genetic and genealogical bases. Cloning and automated HLA-DMB DNA (exons 2, 3 and 4) sequencing have been performed for allele assignation. RESULTS: HLA-DMB*01:01:01 and HLA-DMB*01:03:01 show the highest frequencies. These have been compared to other World wide populations. HLA-DMB*01:03:01 is tightly associated to certain specific HLA-DRB1 alleles in Amerindians. CONCLUSION: The specific Amerindian HLA-DMB allele frequencies and their linkage disequilibrium with other MHC alleles may be crucial to determine HLA-DMB World wide variation, evolution and specific linkage to disease in Amerindians and other populations.
BACKGROUND:HLA-DMB proteins are important for intracellular microbial metabolism in order other major histocompatibility complex (MHC) molecules present peptides to lymphocytes. In addition, HLA-DMB alleles have been found linked to diseases in some ethnic groups and HLA-DMB molecules may be important to explain HLA disease association. OBJECTIVE: To detect HLA-DMB alleles profile in Amerindians for the first time and compare them to other populations. This will establish the bases to study HLA-DMB linkage to disease in Amerindians. METHOD: A group of 168 voluntary Amerindians have been typed for HLA-DMB alleles. They have been characterized both, by genetic and genealogical bases. Cloning and automated HLA-DMB DNA (exons 2, 3 and 4) sequencing have been performed for allele assignation. RESULTS:HLA-DMB*01:01:01 and HLA-DMB*01:03:01 show the highest frequencies. These have been compared to other World wide populations. HLA-DMB*01:03:01 is tightly associated to certain specific HLA-DRB1 alleles in Amerindians. CONCLUSION: The specific Amerindian HLA-DMB allele frequencies and their linkage disequilibrium with other MHC alleles may be crucial to determine HLA-DMB World wide variation, evolution and specific linkage to disease in Amerindians and other populations.
Authors: G V González-Enríquez; B M Torres-Mendoza; J Márquez-Pedroza; M A Macías-Islas; G G Ortiz; J A Cruz-Ramos Journal: Immunogenetics Date: 2018-02-03 Impact factor: 2.846