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Literature DB >> 269437

Separation and infectivity of circular and linear forms of potato spindle tuber viroid.

R A Owens, E Erbe, A Hadidi, R L Steere, T O Diener.

Abstract

Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were found to be infectious.

Entities: Chemical Species

Mesh：

Year: 1977 PMID： 269437 PMCID： PMC431760 DOI： 10.1073/pnas.74.9.3859

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.

Authors: H L Sanger; G Klotz; D Riesner; H J Gross; A K Kleinschmidt
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

2. A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

Authors: H J Vollenweider; J M Sogo; T Koller
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

3. De novo synthesis of potato spindle tuber viroid as measured by incorporation of 32P.

Authors: A Hadidi; T O Diener
Journal: Virology Date: 1977-05-01 Impact factor: 3.616

4. Properties of RNA in formamide.

Authors: J C Pinder; D Z Staynov; W B Gratzer
Journal: Biochemistry Date: 1974-12-17 Impact factor: 3.162

5. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis.

Authors: R W Floyd; M P Stone; W K Joklik
Journal: Anal Biochem Date: 1974-06 Impact factor: 3.365

6. Potato spindle tuber viroid. X. Visualization and size determination by electron microscopy.

Authors: J M Sogo; T Koller; T O Diener
Journal: Virology Date: 1973-09 Impact factor: 3.616

7. Size and secondary structure of potato spindle tuber viroid.

Authors: W L McClements; P Kaesberg
Journal: Virology Date: 1977-02 Impact factor: 3.616

Review 8. Viroids: the smallest known agents of infectious disease.

Authors: T O Diener
Journal: Annu Rev Microbiol Date: 1974 Impact factor: 15.500

9. Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK21 cells.

Authors: L P Villarreal; M Breindl; J J Holland
Journal: Biochemistry Date: 1976-04-20 Impact factor: 3.162

10. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1967-06 Impact factor: 3.162

13 in total

1. Viroid processing: a model involving the central conserved region and hairpin I.

Authors: T O Diener
Journal: Proc Natl Acad Sci U S A Date: 1986-01 Impact factor: 11.205

2. Circular single-stranded RNA replicon in Saccharomyces cerevisiae.

Authors: Y Matsumoto; R Fishel; R B Wickner
Journal: Proc Natl Acad Sci U S A Date: 1990-10 Impact factor: 11.205

3. Molecular cloning and characterization of potato spindle tuber viroid cDNA sequences.

Authors: R A Owens; D E Cress
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

4. Potato spindle tuber and citrus exocortis viroids undergo no major sequence changes during replication in two different hosts.

Authors: E Dickson; T O Diener; H D Robertson
Journal: Proc Natl Acad Sci U S A Date: 1978-02 Impact factor: 11.205

5. Apple scar skin viroid naked RNA is actively transmitted by the whitefly Trialeurodes vaporariorum.

Authors: Yashika Walia; Sunny Dhir; Aijaz Asghar Zaidi; Vipin Hallan
Journal: RNA Biol Date: 2015-09-01 Impact factor: 4.652

6. Viroids and prions.

Authors: T O Diener; M P McKinley; S B Prusiner
Journal: Proc Natl Acad Sci U S A Date: 1982-09 Impact factor: 11.205

7. Studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease A and ribonuclease T1, and modification with bisulfite.

Authors: H Domdey; P Jank; L Sänger; H J Gross
Journal: Nucleic Acids Res Date: 1978-04 Impact factor: 16.971