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Literature DB >> 2693943

Replication of ColE2 and ColE3 plasmids: in vitro replication dependent on plasmid-coded proteins.

Abstract

We developed an in vitro replication system for ColE2 and ColE3 plasmids using cell extracts prepared from bacteria with or without these plasmids. DNA synthesis depended on host DNA polymerase I and was sensitive to rifampicin and chloramphenicol. Preincubation of the extracts with plasmid DNA, however, allowed replication of template DNA added subsequently in a plasmid-specific manner in the presence of rifampicin and chloramphenicol. The plasmid-specified trans-acting factor(s) was detected in cell extracts from bacteria carrying a recombinant plasmid with the region of ColE2 or ColE3 encoding the Rep protein. The plasmid-specified factor(s) consisted at least in part of protein, probably the Rep protein. In vitro replication started within a region of ColE2 or ColE3 containing the smallest cis-acting segment essential for in vivo replication and proceeded in a fixed direction.

Entities: Chemical

Mesh：

Substances：

Year: 1989 PMID： 2693943 DOI： 10.1007/bf00261184

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

17 in total

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Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

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Journal: Mol Gen Genet Date: 1988-05

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Journal: Mol Gen Genet Date: 1988-11

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Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

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Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

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17 in total

1. Specific interaction between the initiator protein (Rep) and origin of plasmid ColE2-P9.

Authors: M Han; M Yagura; T Itoh
Journal: J Bacteriol Date: 2006-11-10 Impact factor: 3.490

2. Control of ColE2 DNA replication: in vitro binding of the antisense RNA to the Rep mRNA.

Authors: T Sugiyama; T Itoh
Journal: Nucleic Acids Res Date: 1993-12-25 Impact factor: 16.971

3. Initiation of unidirectional ColE2 DNA replication by a unique priming mechanism.

Authors: S Takechi; T Itoh
Journal: Nucleic Acids Res Date: 1995-10-25 Impact factor: 16.971

4. Control of ColE2 plasmid replication: regulation of Rep expression by a plasmid-coded antisense RNA.

Authors: S Takechi; H Yasueda; T Itoh
Journal: Mol Gen Genet Date: 1994-07-08

5. Structural basis for replication origin unwinding by an initiator primase of plasmid ColE2-P9: duplex DNA unwinding by a single protein.

Authors: Hiroshi Itou; Masaru Yagura; Yasuo Shirakihara; Tateo Itoh
Journal: J Biol Chem Date: 2014-12-23 Impact factor: 5.157

6. Anatomy of the replication origin of plasmid ColE2-P9.

Authors: Masaru Yagura; Shin-Ya Nishio; Hideki Kurozumi; Cheng-Fu Wang; Tateo Itoh
Journal: J Bacteriol Date: 2006-02 Impact factor: 3.490

7. Control of ColE2 plasmid replication: negative regulation of the expression of the plasmid-specified initiator protein, Rep, at a posttranscriptional step.

Authors: H Yasueda; S Takechi; T Sugiyama; T Itoh
Journal: Mol Gen Genet Date: 1994-07-08