Ilya V Lyagin1,2, Mariia S Andrianova3, Elena N Efremenko4,5. 1. Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 1, building 3, Moscow, 119991, Russia. 2. Institute of Biochemical Physics RAS, Kosygin St. 4, Moscow, 119334, Russia. 3. SMC Technological Center MIET, Proezd No. 4806, 5, Moscow, Zelenograd, 124498, Russia. 4. Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 1, building 3, Moscow, 119991, Russia. elena_efremenko@list.ru. 5. Institute of Biochemical Physics RAS, Kosygin St. 4, Moscow, 119334, Russia. elena_efremenko@list.ru.
Abstract
The catalytic activity of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C-P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.
The catalytic activity of hexahistidine-tagged pan class="Chemical">organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C-P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.