Jodel D Pearson1, Jingxi Zhang1, Zuoqiao Wu1, Kayla D Thew1, Katelynn J Rowe1, Julinor T C Bacani2, Robert J Ingham3. 1. Department of Medical Microbiology and Immunology and Li Ka Shing Institute of Virology, University of Alberta, Katz Group Centre for Pharmacy and Health Research, University of Alberta, Edmonton, Canada. 2. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada. 3. Department of Medical Microbiology and Immunology and Li Ka Shing Institute of Virology, University of Alberta, Katz Group Centre for Pharmacy and Health Research, University of Alberta, Edmonton, Canada. ringham@ualberta.ca.
Unfortunately, the original version of this article [1] contained an error. A figure was mislabelled. In Figs. 5c and d the Doxorubicin (Doxo) concentrations should be in μM. Here is most recent version of the figure with it correctly labelled.From: Expression of granzyme B sensitizes ALK + ALCL tumour cells to apoptosis-inducing drugs. GzB knock-down reduces the sensitivity of ALK + ALCL cell lines to doxorubicin-induced apoptosis. SUP-M2 (a) or SR (b) cells expressing either control or GzB targeting shRNA were left untreated (untr.) or were treated with indicated concentrations of doxorubicin (Doxo) for 12 h at 37 °C. Cells were then lysed and lysates were probed with an anti-PARP antibody. The arrow indicates cleaved PARP. The anti-β-actin blot demonstrates equivalent protein loading. The percent cleaved PARP (% cleaved PARP) was determined by densitometry and represents the percentage of cleaved PARP as a fraction of total PARP. Molecular mass standards are indicated to the left of the western blots. SUP-M2 (c) or SR (d) cells expressing either control or GzB shRNA were left untreated (DMSO) or were treated with the indicated concentrations of doxorubicin (doxo) for 12 h at 37 °C. DNA fragmentation was then examined by TUNEL staining and results were expressed as the percentage of TUNEL-positive cells. The results shown represent the mean and standard deviation of 4 independent experiments. p values comparing cells expressing GzB shRNA to cells expressing control shRNA were obtained by performing paired, one-tailed t-tests. *p < 0.05, §p < 0.01, #p < 0.005, δp < 0.001
Authors: Joel D Pearson; Jingxi Zhang; Zuoqiao Wu; Kayla D Thew; Katelynn J Rowe; Julinor T C Bacani; Robert J Ingham Journal: Mol Cancer Date: 2014-08-29 Impact factor: 27.401