Karla de Faria Vasconcelos1, Lívia Dos Santos Corpas2, Bernardo Mattos da Silveira3, Kjell Laperre4, Luis Eduardo Padovan5, Reinhilde Jacobs6,7, Paulo Henrique Luiz de Freitas8, Ivo Lambrichts9, Frab Norberto Bóscolo1. 1. Department of Oral Diagnosis, Division of Oral Radiology, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil. 2. Department of Prosthetic Dentistry, Oral Health Sciences, Faculty of Medicine, Katholieke Universiteit Leuven, Leuven, Belgium. 3. Department of Prosthetic Dentistry, Brazilian Dentistry Association (ABO-RJ), Rio de Janeiro, Brazil. 4. Bruker/Skyscan, Kontich, Belgium. 5. Department of Implant Dentistry, Latin American Institute for Dental Research and Training (ILAPEO), Curitiba, Brazil. 6. OMFS Impath Research Group, Department of Imaging & Pathology, Faculty of Medicine, University of Leuven, Leuven, Belgium. 7. Department of Maxillofacial Surgery, Leuven University Hospital, Leuven, Belgium. 8. Department of Dentistry, Federal University of Sergipe, Lagarto, Brazil. 9. Biomedical Research Institute, Laboratory of Morphology, Hasselt University, Diepenbeek, Belgium.
Abstract
PURPOSE: To assess bone microarchitecture in maxillary sites grafted with autogenous or xenogenous grafts as well as to demonstrate the usefulness of microCT in dental implant research. MATERIALS AND METHODS: Samples (n = 12) consisting of titanium fixation screws covered by at least 0.5-1 mm of human bone were obtained from 17 sites grafted with autogenous or xenogenous materials and prepared for microCT scanning and conventional histology. Bone histomorphometric parameters were evaluated in three distinct regions (graft region, transitional region, and native bone region). Three-dimensional (3D) bone-to-implant contact (BIC) calculation was performed using microCT data. Histological sections were used to calculate two-dimensional (2D) BIC percentages, which were compared with values obtained from 2D microCT images. RESULTS: Histomorphometric parameters varied according to the type of graft used, but sites reconstructed with autogenous bone showed higher mean values in general. In autograft samples, indices for parameters such as Tb.Th and Tb.Sp were significantly different when the native bone region was compared to the graft region. While a higher mean 3D BIC was found in the native bone region for both graft materials, significant BIC differences were absent when graft types were compared. The 2D BIC percentages obtained from histological and microCT images were similar. CONCLUSIONS: Autografts outperformed the xenogenous material used in this study concerning the histomorphometric parameters assessed. While graft type did not seem to influence 3D BIC, the native bone region showed the highest BIC percentages when compared to the other regions in both graft groups. In addition, 2D BIC ratios were similar regardless of graft material or image source (histological sections x microCT slices). Taken together, our findings suggest that microCT is an effective tool for 2D and 3D histomorphometric and BIC assessments in dental implant research.
PURPOSE: To assess bone microarchitecture in maxillary sites grafted with autogenous or xenogenous grafts as well as to demonstrate the usefulness of microCT in dental implant research. MATERIALS AND METHODS: Samples (n = 12) consisting of titanium fixation screws covered by at least 0.5-1 mm of human bone were obtained from 17 sites grafted with autogenous or xenogenous materials and prepared for microCT scanning and conventional histology. Bone histomorphometric parameters were evaluated in three distinct regions (graft region, transitional region, and native bone region). Three-dimensional (3D) bone-to-implant contact (BIC) calculation was performed using microCT data. Histological sections were used to calculate two-dimensional (2D) BIC percentages, which were compared with values obtained from 2D microCT images. RESULTS: Histomorphometric parameters varied according to the type of graft used, but sites reconstructed with autogenous bone showed higher mean values in general. In autograft samples, indices for parameters such as Tb.Th and Tb.Sp were significantly different when the native bone region was compared to the graft region. While a higher mean 3D BIC was found in the native bone region for both graft materials, significant BIC differences were absent when graft types were compared. The 2D BIC percentages obtained from histological and microCT images were similar. CONCLUSIONS: Autografts outperformed the xenogenous material used in this study concerning the histomorphometric parameters assessed. While graft type did not seem to influence 3D BIC, the native bone region showed the highest BIC percentages when compared to the other regions in both graft groups. In addition, 2D BIC ratios were similar regardless of graft material or image source (histological sections x microCT slices). Taken together, our findings suggest that microCT is an effective tool for 2D and 3D histomorphometric and BIC assessments in dental implant research.
Authors: J Ciaran Hutchinson; Susan C Shelmerdine; Ian C Simcock; Neil J Sebire; Owen J Arthurs Journal: Br J Radiol Date: 2017-05-04 Impact factor: 3.039
Authors: Dandan Song; Sohaib Shujaat; Yan Huang; Jeroen Van Dessel; Constantinus Politis; Ivo Lambrichts; Reinhilde Jacobs Journal: Int J Implant Dent Date: 2021-02-18