| Literature DB >> 26930613 |
Sung Min Kim1, Kyung Tae Lim2, Tae Hwan Kwak2, Seung Chan Lee2, Jung Hyun Im2, Sai Hali2, Seon In Hwang2, Dajeong Kim3, Jeongho Hwang4, Kee-Pyo Kim5, Hak-Jae Chung6, Jeong Beom Kim7, Kinarm Ko8, Hyung-Min Chung8, Hoon Taek Lee4, Hans R Schöler9, Dong Wook Han10.
Abstract
Somatic cells could be directly converted into induced neural stem cells (iNSCs) by ectopic expression of defined transcription factors. However, the underlying mechanism of direct lineage transition into iNSCs is largely unknown. In this study, we examined the effect of genetic background on the direct conversion process into an iNSC state. The iNSCs from two different mouse strains exhibited the distinct efficiency of lineage conversion as well as clonal expansion. Furthermore, the expression levels of endogenous NSC markers, silencing of transgenes, and in vitro differentiation potential were also different between iNSC lines from different strains. Therefore, our data suggest that the genetic background of starting cells influences the conversion efficiency as well as reprogramming status of directly converted iNSCs.Entities:
Keywords: Cell fate transition; Direct conversion; Direct reprogramming; Genetic backgrounds; iNSCs
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Year: 2016 PMID： 26930613 DOI： 10.1016/j.scr.2016.02.025
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020