INTRODUCTION: Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in women, and undetected cases of the disease are highly associated with long-term complications. Despite the high prevalence of infections in Brazil, very little is known about the distribution of C. trachomatis genovars. In this study, we determined the prevalence and genotypes of C. trachomatis in women treated at a public hospital in the Brazilian city of Belém, the capital of the state of Pará. METHODOLOGY: A total of 154 women were tested for chlamydial infection by PCR using specific primers for the C. trachomatis cryptic plasmid. Genotyping of positive samples was performed by sequencing the ompA gene and conducting further phylogenetic analysis. RESULTS: Out of the 154 samples, 17 were found to be positive using C. trachomatis cryptic plasmid PCR. The overall prevalence of C. trachomatis infection was 11%, with the highest prevalence observed in women between 16 and 20 years of age. Five genotypes were found to be associated with endocervical infection. Genotype F was most frequently found (37.5%), followed by genotypes J (25%), E (25%), I (6.25%), and D (6.25%). CONCLUSIONS: This study emphasizes the relevance of C. trachomatis infection in the young female population of the Brazilian Amazon region. It also demonstrates the diversity of genotypes involved in genital infection in this population.
INTRODUCTION: Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in women, and undetected cases of the disease are highly associated with long-term complications. Despite the high prevalence of infections in Brazil, very little is known about the distribution of C. trachomatis genovars. In this study, we determined the prevalence and genotypes of C. trachomatis in women treated at a public hospital in the Brazilian city of Belém, the capital of the state of Pará. METHODOLOGY: A total of 154 women were tested for chlamydial infection by PCR using specific primers for the C. trachomatis cryptic plasmid. Genotyping of positive samples was performed by sequencing the ompA gene and conducting further phylogenetic analysis. RESULTS: Out of the 154 samples, 17 were found to be positive using C. trachomatis cryptic plasmid PCR. The overall prevalence of C. trachomatis infection was 11%, with the highest prevalence observed in women between 16 and 20 years of age. Five genotypes were found to be associated with endocervical infection. Genotype F was most frequently found (37.5%), followed by genotypes J (25%), E (25%), I (6.25%), and D (6.25%). CONCLUSIONS: This study emphasizes the relevance of C. trachomatis infection in the young female population of the Brazilian Amazon region. It also demonstrates the diversity of genotypes involved in genital infection in this population.
Authors: Leonardo Miranda Dos Santos; Josiellem Damasceno de Souza; Hilary Acha Mbakwa; Akim Felipe Santos Nobre; Rodrigo Covre Vieira; Stephen Francis Ferrari; Anderson Raiol Rodrigues; Edna Aoba Yassui Ishikawa; João Farias Guerreiro; Maísa Silva de Sousa Journal: PLoS One Date: 2022-07-18 Impact factor: 3.752
Authors: Luana L S Rodrigues; Justin Hardick; Alcina F Nicol; Mariza G Morgado; Katrini G Martinelli; Vanessa S de Paula; José H Pilotto; Charlotte A Gaydos Journal: PLoS One Date: 2019-04-23 Impact factor: 3.752