Raluca Elena Sandu1, Adriana Uzoni2, Ovidiu Ciobanu1,3, Mihai Moldovan4, Andrei Anghel5, Eugen Radu6, Andrew N Coogan7, Aurel Popa-Wagner2,1. 1. University of Medicine and Pharmacy, Craiova, Romania. 2. Molecular Psychiatry, Department of Psychiatry, University of Medicine Rostock, Rostock, Germany. 3. Department of Psychiatry, University Medicine of Saarland, Homburg/Saar, Germany. 4. Neuroscience and Pharmacology, Panum, University of Copenhagen, Copenhagen, Denmark. 5. Department of Biochemistry, University of Medicine and Pharmacy "Victor Babes", Timisoara, Romania. 6. University of Medicine and Pharmacy Carol Davila, Bucharest, Romania. 7. Department of Psychology, Maynooth University, National University of Ireland, Maynooth, Ireland.
Abstract
PURPOSE: In aged humans, stroke is a major cause of disability for which no neuroprotective measures are available. In animal studies of focal ischemia, short-term hypothermia often reduces infarct size. Nevertheless, efficient neuroprotection requires long-term, regulated lowering of whole body temperature. Previously, we reported that post-stroke exposure to hydrogen sulfide (H2S) effectively lowers whole body temperature and confers neuroprotection in aged animals. METHODS: In the present study using behavioral tests, MRI, telemetrical EEG, BP and temperature recordings, RT-PCR and immunofluorescence, we assessed infarct size, vascular density, neurogenesis and as well as the expression of genes coding for proteasomal proteins as well as in post-stroke aged Sprague-Dawley rats exposed to H2S- induced hypothermia. RESULTS: Two days exposure to mild hypothermia diminishes the expression of several genes involved in protein degradation, thereby leading to better preservation of infarcted tissue. Further, hypothermia increased the density of newly formed blood vessels in the peri-lesional cortex did not enhance neurogenesis in the infarcted area of aged rats. Likewise, there was improved recovery of fine vestibulomotor function and asymmetric sensorimotor deficit. CONCLUSION: Long-term hypothermia may be a viable clinical approach by simultaneously targeting multiple processes including better tissue preservation, enhanced vascular density and improved behavioral performance.
PURPOSE: In aged humans, stroke is a major cause of disability for which no neuroprotective measures are available. In animal studies of focal ischemia, short-term hypothermia often reduces infarct size. Nevertheless, efficient neuroprotection requires long-term, regulated lowering of whole body temperature. Previously, we reported that post-stroke exposure to hydrogen sulfide (H2S) effectively lowers whole body temperature and confers neuroprotection in aged animals. METHODS: In the present study using behavioral tests, MRI, telemetrical EEG, BP and temperature recordings, RT-PCR and immunofluorescence, we assessed infarct size, vascular density, neurogenesis and as well as the expression of genes coding for proteasomal proteins as well as in post-stroke aged Sprague-Dawley rats exposed to H2S- induced hypothermia. RESULTS: Two days exposure to mild hypothermia diminishes the expression of several genes involved in protein degradation, thereby leading to better preservation of infarcted tissue. Further, hypothermia increased the density of newly formed blood vessels in the peri-lesional cortex did not enhance neurogenesis in the infarcted area of aged rats. Likewise, there was improved recovery of fine vestibulomotor function and asymmetric sensorimotor deficit. CONCLUSION: Long-term hypothermia may be a viable clinical approach by simultaneously targeting multiple processes including better tissue preservation, enhanced vascular density and improved behavioral performance.
Entities:
Keywords:
Aging; H2S; hypothermia; neurogenesis; proteasome; stroke; vascular density
Authors: Lucy A Murtha; Daniel J Beard; Julia T Bourke; Debbie Pepperall; Damian D McLeod; Neil J Spratt Journal: Front Aging Neurosci Date: 2016-05-27 Impact factor: 5.750