Baljit K Saundh1, Richard Baker2, Mark Harris3, Antony Hale2. 1. Leeds Teaching Hospital NHS Trust, Microbiology and Renal Unit, Leeds, United Kingdom. Electronic address: Baljit.Saundh@nhs.net. 2. Leeds Teaching Hospital NHS Trust, Microbiology and Renal Unit, Leeds, United Kingdom. 3. School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom.
Abstract
BACKGROUND: Both JC polyomavirus (JCPyV) and BK polyomavirus (BKPyV) are acquired at an early age. JCPyV causes progressive multifocal leukoencephalopathy and has been described in association with nephropathy. OBJECTIVES: Urine and plasma samples from renal transplant recipients (RTRs) were examined for JCPyV to determine its involvement in causing infection and disease. STUDY DESIGN: JCPyV testing was performed on 112 RTRs included in a randomised controlled study of steroid-sparing immunosuppressive regimens [1]. Urine and EDTA blood samples were collected pre- and post-transplantation and analysed for JCPyV using real-time PCR and sequencing to determine genotype and viral variation. Donor and recipient IgG antibody status to JCPyV was also determined. RESULTS: Overall, 13.3% of RTRs were positive for JCPyV of which one patient developed viraemia without viruria. JCPyV DNA was detected early following transplantation (defined as five days post transplantation) from recipients with donors that were positive for JCPyV IgG antibodies. No dual cases of JCPyV and BKPyV were observed. One patient sample had sequence duplication in the non-coding control region. CONCLUSIONS: Like BKPyV, JCPyV tends to occur early post transplantation but did not result in sustained viraemia. There was no deterioration of renal function in patients positive for JCPyV. As with other viruses, JCPyV donor serostatus was a risk factor for detection of JCPyV DNA. JCPyV appears to protect individuals from BKPyV infection, as recipients were twice as likely to develop BKPyV with a negative JCPyV donor.
BACKGROUND: Both JC polyomavirus (JCPyV) and BK polyomavirus (BKPyV) are acquired at an early age. JCPyV causes progressive multifocal leukoencephalopathy and has been described in association with nephropathy. OBJECTIVES: Urine and plasma samples from renal transplant recipients (RTRs) were examined for JCPyV to determine its involvement in causing infection and disease. STUDY DESIGN:JCPyV testing was performed on 112 RTRs included in a randomised controlled study of steroid-sparing immunosuppressive regimens [1]. Urine and EDTA blood samples were collected pre- and post-transplantation and analysed for JCPyV using real-time PCR and sequencing to determine genotype and viral variation. Donor and recipient IgG antibody status to JCPyV was also determined. RESULTS: Overall, 13.3% of RTRs were positive for JCPyV of which one patient developed viraemia without viruria. JCPyV DNA was detected early following transplantation (defined as five days post transplantation) from recipients with donors that were positive for JCPyV IgG antibodies. No dual cases of JCPyV and BKPyV were observed. One patient sample had sequence duplication in the non-coding control region. CONCLUSIONS: Like BKPyV, JCPyV tends to occur early post transplantation but did not result in sustained viraemia. There was no deterioration of renal function in patients positive for JCPyV. As with other viruses, JCPyVdonor serostatus was a risk factor for detection of JCPyV DNA. JCPyV appears to protect individuals from BKPyVinfection, as recipients were twice as likely to develop BKPyV with a negative JCPyVdonor.
Authors: Britta Höcker; Julia Tabatabai; Lukas Schneble; Jun Oh; Florian Thiel; Lars Pape; Krisztina Rusai; Rezan Topaloglu; Birgitta Kranz; Günter Klaus; Nikoleta Printza; Onder Yavascan; Alexander Fichtner; Kai Krupka; Thomas Bruckner; Rüdiger Waldherr; Michael Pawlita; Paul Schnitzler; Hans H Hirsch; Burkhard Tönshoff Journal: Pediatr Nephrol Date: 2018-07-30 Impact factor: 3.714
Authors: Sara Querido; Carolina Ormonde; Teresa Adragão; Inês Costa; Maria Ana Pessanha; Perpétua Gomes; André Weigert Journal: Int J Nephrol Date: 2021-08-19