Cui Zhang1, Yanyan Zhou1, Yajie Zhou1, Ying Lu2, Danfeng Wang1. 1. a College of Pharmacy , Harbin University of Commerce , Harbin , China ; 2. b Heilongjiang Songhuajiang Pharmaceutical Limited , Harbin , China.
Abstract
PURPOSE: This study was performed to assess the effect of resveratrol on the expression of eukaryotic initiation factor 2α (eIF2α) and activating transcription factor 4 (ATF4) in renal tissues of rats with unilateral ureteral obstruction (UUO). METHODS: Using UUO animal model, after 14 days of surgery, pathological changes were detected by HE staining, renal tubular damage index, renal interstitial collagen deposition area were evaluated by Masson staining, in situ cell apoptosis in renal tissue was analyzed by TUNEL assay, and protein expression of eIF2α and ATF4 in renal tissue was analyzed using western blot detection. RESULTS: After comparison of the treatment groups with model group, we observed that the degree of renal tubular damage, relative area of renal interstitial collagen and eIF2α, ATF4 protein expression were also significantly reduced (p<0.05, p <0.01) in the high-dose resveratrol group. CONCLUSION: Resveratrol can reduce the level of eIF2α protein expression, which further reduces the ATF4 levels.
PURPOSE: This study was performed to assess the effect of resveratrol on the expression of eukaryotic initiation factor 2α (eIF2α) and activating transcription factor 4 (ATF4) in renal tissues of rats with unilateral ureteral obstruction (UUO). METHODS: Using UUO animal model, after 14 days of surgery, pathological changes were detected by HE staining, renal tubular damage index, renal interstitial collagen deposition area were evaluated by Masson staining, in situ cell apoptosis in renal tissue was analyzed by TUNEL assay, and protein expression of eIF2α and ATF4 in renal tissue was analyzed using western blot detection. RESULTS: After comparison of the treatment groups with model group, we observed that the degree of renal tubular damage, relative area of renal interstitial collagen and eIF2α, ATF4 protein expression were also significantly reduced (p<0.05, p <0.01) in the high-dose resveratrol group. CONCLUSION:Resveratrol can reduce the level of eIF2α protein expression, which further reduces the ATF4 levels.
Authors: Sin Young Choi; Zhe Hao Piao; Li Jin; Jung Ha Kim; Gwi Ran Kim; Yuhee Ryu; Ming Quan Lin; Hyung-Seok Kim; Hae Jin Kee; Myung Ho Jeong Journal: PLoS One Date: 2016-11-30 Impact factor: 3.240