OBJECTIVES: Immunohistochemistry (IHC) is an important component of lung cancer diagnosis and management, although performance can be limited due to tissue availability. We describe a novel technique for processing brush-tip washings (BTW) and evaluate the feasibility of IHC testing on these samples. STUDY DESIGN: All patients who had cell blocks (CB) created from BTW following bronchoscopic investigation of peripheral lung lesions were included. CB were assessed for adequate material before undergoing IHC staining. RESULTS: 75 patients were included in the study, with bronchoscopic diagnosis of malignancy achieved in 77%. Sixty-seven samples (89%) had sufficient cells for diagnosis on CB and 56 of these (84%) proved amenable to IHC. CB created from BTW were the sole specimens available for IHC subtyping in 7 patients (9%). CONCLUSIONS: CB are easily created from BTW and are a simple method for increasing the diagnostic utility of bronchoscopic specimens without increasing the risk or duration of bronchoscopy. IHC can be easily performed in a high proportion of cases, increasing the likelihood of accurate sub-typing of tumours following diagnostic bronchoscopy.
OBJECTIVES: Immunohistochemistry (IHC) is an important component of lung cancer diagnosis and management, although performance can be limited due to tissue availability. We describe a novel technique for processing brush-tip washings (BTW) and evaluate the feasibility of IHC testing on these samples. STUDY DESIGN: All patients who had cell blocks (CB) created from BTW following bronchoscopic investigation of peripheral lung lesions were included. CB were assessed for adequate material before undergoing IHC staining. RESULTS: 75 patients were included in the study, with bronchoscopic diagnosis of malignancy achieved in 77%. Sixty-seven samples (89%) had sufficient cells for diagnosis on CB and 56 of these (84%) proved amenable to IHC. CB created from BTW were the sole specimens available for IHC subtyping in 7 patients (9%). CONCLUSIONS: CB are easily created from BTW and are a simple method for increasing the diagnostic utility of bronchoscopic specimens without increasing the risk or duration of bronchoscopy. IHC can be easily performed in a high proportion of cases, increasing the likelihood of accurate sub-typing of tumours following diagnostic bronchoscopy.
Authors: Asha Bonney; Michael Christie; Anne Beaty; Sebastian Lunke; Graham Taylor; Louis Irving; Daniel Steinfort Journal: J Thorac Dis Date: 2016-09 Impact factor: 2.895
Authors: Insu Kim; Jung Seop Eom; Ah Rong Kim; Chang Hun Lee; Geewon Lee; Eun Jung Jo; Mi-Hyun Kim; Jeong Ha Mok; Kwangha Lee; Ki Uk Kim; Hye-Kyung Park; Min Ki Lee Journal: PLoS One Date: 2019-02-26 Impact factor: 3.240
Authors: Steven Bozinovski; Amanda Vannitamby; Kanishka Rangamuwa; Savreet Aujla; Hao Wang; Christian Aloe; Louis Irving; Tracy T Leong; Daniel P Steinfort Journal: Transl Lung Cancer Res Date: 2021-06