OBJECTIVE: The primary purpose of this paper is to investigate the relationship between the microRNA 146a (miR-146a) and the proliferation of cells occurring in glioblastoma multiforme. The secondary purpose of the paper is to investigate abnormalities of expression in miR-146a. METHODS: A real-time PCR assay was used to investigate the abnormal expression of miR-146a in glioma and adjacent tissue. Lipofection was used to transfect a mimic of miR-146a and induce the upregulation of miR-146a. Real-time PCR was used to observe the expression level of miR-146a. A cell viability analysis was conducted using MTT. A luciferase report vector was used to identify potential targets for miR-146a. RESULTS: The miR-146a component was found to be downregulated in glioma tissue compared with adjacent nontumor tissue (p<0.05). The upregulation of miR-146a in glioma cells through miR-146a mimic transfection led to reduction of cell viability and to an increase in the percentage of apoptosis. Notch1 was the name of the potential targeted gene for miR-146a in glioma. CONCLUSIONS: The study found that the presence of miR-146a potentially affected the proliferation of glioma cells by regulating the rate of Notch1 expression.
OBJECTIVE: The primary purpose of this paper is to investigate the relationship between the microRNA 146a (miR-146a) and the proliferation of cells occurring in glioblastoma multiforme. The secondary purpose of the paper is to investigate abnormalities of expression in miR-146a. METHODS: A real-time PCR assay was used to investigate the abnormal expression of miR-146a in glioma and adjacent tissue. Lipofection was used to transfect a mimic of miR-146a and induce the upregulation of miR-146a. Real-time PCR was used to observe the expression level of miR-146a. A cell viability analysis was conducted using MTT. A luciferase report vector was used to identify potential targets for miR-146a. RESULTS: The miR-146a component was found to be downregulated in glioma tissue compared with adjacent nontumor tissue (p&lt;0.05). The upregulation of miR-146a in glioma cells through miR-146a mimic transfection led to reduction of cell viability and to an increase in the percentage of apoptosis. Notch1 was the name of the potential targeted gene for miR-146a in glioma. CONCLUSIONS: The study found that the presence of miR-146a potentially affected the proliferation of glioma cells by regulating the rate of Notch1 expression.
Authors: Juan de Los Santos-Jiménez; José A Campos-Sandoval; Clara Márquez-Torres; Nieves Urbano-Polo; David Brøndegaard; Mercedes Martín-Rufián; Carolina Lobo; Ana Peñalver; María C Gómez-García; Janet Martín-Campos; Carolina Cardona; Laura Castilla; Felipe da Costa Souza; Tzuling Cheng; Juan A Segura; Francisco J Alonso; Rui Curi; Alison Colquhoun; Ralph J DeBerardinis; Javier Márquez; José M Matés Journal: J Biomed Sci Date: 2021-02-20 Impact factor: 8.410