L Chen1, X Feng2, Y Wang3, X Xu3, C Wan2, J Wang4, H Mu5. 1. Department of Clinical Laboratory, The First Central Hospital of Tianjin, Tianjin, China. 2. Department of Neurosurgery, The First Central Hospital of Tianjin, Tianjin, China. 3. Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin, China. 4. Department of Transplantation Surgery, The First Central Hospital of Tianjin, Tianjin, China. 5. Department of Clinical Laboratory, The First Central Hospital of Tianjin, Tianjin, China. Electronic address: 2356677342@qq.com.
Abstract
BACKGROUND: From the medical and ethical points of view, donation after brain death is a more acceptable organ source than that from a living donor because it has the advantage of providing multiple organs from a single donor source. Hence, it has become a more promising field of research which focuses on the protection of organs at brain death Here we investigated the role of transforming growth factor (TGF)-β1 in a porcine model of brain death. METHODS: A porcine model of brain death was established by increasing the intracranial pressure (ICP) after which TGF-β1 was monitored by immunofluorescence at the following time points: before ICP was performed (t1), at brain death (t2), and at 3 (t3), 6 (t4), 9 (t5), and 18 (t6) hours after brain death. The data were analyzed using the fixed effect regression method and the correlation between the results was determined by Pearson analysis. RESULTS: Our results showed that there was a significant increase in the levels of TGF-β1 (P < .05), urea (P < .01), creatinine (P < .01), and aspartate aminotransferase (AST; P < .01) during the 18-hour brain death process. There were negative correlations between TGF-β1 and urea, creatinine, alanine aminotransferase, AST, and total bilirubin. The negative correlations between TGF-β1 and creatinine and AST achieved statistical significance (P < .05). CONCLUSIONS: These findings taken together confirm that significant damages are caused to the myocardial fiber cell and kidney glomerulus during brain death process, and that TGF-β1 is associated with the protection of these organs.
BACKGROUND: From the medical and ethical points of view, donation after brain death is a more acceptable organ source than that from a living donor because it has the advantage of providing multiple organs from a single donor source. Hence, it has become a more promising field of research which focuses on the protection of organs at brain death Here we investigated the role of transforming growth factor (TGF)-β1 in a porcine model of brain death. METHODS: A porcine model of brain death was established by increasing the intracranial pressure (ICP) after which TGF-β1 was monitored by immunofluorescence at the following time points: before ICP was performed (t1), at brain death (t2), and at 3 (t3), 6 (t4), 9 (t5), and 18 (t6) hours after brain death. The data were analyzed using the fixed effect regression method and the correlation between the results was determined by Pearson analysis. RESULTS: Our results showed that there was a significant increase in the levels of TGF-β1 (P < .05), urea (P < .01), creatinine (P < .01), and aspartate aminotransferase (AST; P < .01) during the 18-hour brain death process. There were negative correlations between TGF-β1 and urea, creatinine, alanine aminotransferase, AST, and total bilirubin. The negative correlations between TGF-β1 and creatinine and AST achieved statistical significance (P < .05). CONCLUSIONS: These findings taken together confirm that significant damages are caused to the myocardial fiber cell and kidney glomerulus during brain death process, and that TGF-β1 is associated with the protection of these organs.
Authors: Tiffany J Zens; Juan S Danobeitia; Peter J Chlebeck; Laura J Zitur; Scott Odorico; Kevin Brunner; Jennifer Coonen; Saverio Capuano; Anthony M D'Alessandro; Kristina Matkowskyj; Weixiong Zhong; Jose Torrealba; Luis Fernandez Journal: PLoS One Date: 2017-09-19 Impact factor: 3.240
Authors: Felix Poppelaars; Mariana Gaya da Costa; Bernardo Faria; Siawosh K Eskandari; Jeffrey Damman; Marc A Seelen Journal: Clin Kidney J Date: 2021-09-17