Hyun Jin Jo1, Jaeyeon Kim2, Nayoung Kim3,4, Ji Hyun Park2, Ryoung Hee Nam1, Yeong-Jae Seok5, Yeon-Ran Kim5, Joo Sung Kim2, Jung Mogg Kim6, Jung Min Kim7, Dong Ho Lee1,2, Hyun Chae Jung1. 1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. 2. Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea. 3. Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. nayoungkim49@empas.com. 4. Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea. nayoungkim49@empas.com. 5. Department of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Korea. 6. Department of Microbiology, College of Medicine, Hanyang University, Seoul, Korea. 7. NAR Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, South Korea.
Abstract
BACKGROUND: Little is known about the role of gastric microbiota except for Helicobacter pylori (HP) in human health and disease. We compared the differences of human gastric microbiota according to gastric cancer or control and HP infection status and assessed the role of bacteria other than HP. METHODS: Gastric microbiota of 63 antral mucosal and 18 corpus mucosal samples were analyzed by bar-coded 454 pyrosequencing of the 16S rRNA gene. Antral samples were divided into four subgroups based on HP positivity in pyrosequencing and the presence of cancer. The analysis was focused on bacteria other than HP, especially nitrosating or nitrate-reducing bacteria (NB). The changes of NB in antral mucosa of 16 subjects were followed up. RESULTS: The number of NB other than HP (non-HP-NB) was two times higher in the cancer groups than in the control groups, but it did not reach statistical significance. The number of non-HP-NB tends to increase over time, but this phenomenon was prevented by HP eradication in the HP-positive control group, but not in the HP-positive cancer group. CONCLUSION: We could not find the significant role of bacteria other than HP in the gastric carcinogenesis.
BACKGROUND: Little is known about the role of gastric microbiota except for Helicobacter pylori (HP) in human health and disease. We compared the differences of human gastric microbiota according to gastric cancer or control and HP infection status and assessed the role of bacteria other than HP. METHODS: Gastric microbiota of 63 antral mucosal and 18 corpus mucosal samples were analyzed by bar-coded 454 pyrosequencing of the 16S rRNA gene. Antral samples were divided into four subgroups based on HP positivity in pyrosequencing and the presence of cancer. The analysis was focused on bacteria other than HP, especially nitrosating or nitrate-reducing bacteria (NB). The changes of NB in antral mucosa of 16 subjects were followed up. RESULTS: The number of NB other than HP (non-HP-NB) was two times higher in the cancer groups than in the control groups, but it did not reach statistical significance. The number of non-HP-NB tends to increase over time, but this phenomenon was prevented by HP eradication in the HP-positive control group, but not in the HP-positive cancer group. CONCLUSION: We could not find the significant role of bacteria other than HP in the gastric carcinogenesis.
Authors: Kaisa Thorell; Johan Bengtsson-Palme; Oscar Hsin-Fu Liu; Reyna Victoria Palacios Gonzales; Intawat Nookaew; Linda Rabeneck; Lawrence Paszat; David Y Graham; Jens Nielsen; Samuel B Lundin; Åsa Sjöling Journal: Infect Immun Date: 2017-09-20 Impact factor: 3.441
Authors: Heikki Huhta; Olli Helminen; Joonas H Kauppila; Tuula Salo; Katja Porvari; Juha Saarnio; Petri P Lehenkari; Tuomo J Karttunen Journal: J Histochem Cytochem Date: 2016-07-01 Impact factor: 2.479