BACKGROUND: Gastrointestinal (GI) and non-GI disorders are associated with altered intestinal permeability, which can be measured in vivo by urinary excretion after oral lactulose and mannitol ingestion. Inadvertent dietary consumption of (12) Carbon ((12) C, regular) mannitol in food or from other sources may interfere with the test's interpretation. (13) Carbon ((13) C) constitutes 1% of carbon in nature and (13) C mannitol is a stable isotope. Our aim was to determine the performance of (13) C mannitol for measurement of intestinal permeability. METHODS: Ten healthy volunteers underwent intestinal permeability assay using coadministered (12) C mannitol, (13) C mannitol and lactulose, followed by timed urine collections. Urinary sugar concentrations were measured using tandem high performance liquid chromatography-mass spectrometry. KEY RESULTS: We found that (13) C mannitol can be distinguishable from (12) C mannitol on tandem mass spectrometry. In addition, (13) C mannitol had ~20-fold lower baseline contamination compared to (12) C mannitol. We describe here the (13) C mannitol assay method for the measurement of intestinal permeability. CONCLUSIONS & INFERENCES: In conclusion, (13) C mannitol is superior to (12) C mannitol for measurement of intestinal permeability. It avoids issues with baseline contamination and erratic excretions during the testing period.
BACKGROUND:Gastrointestinal (GI) and non-GI disorders are associated with altered intestinal permeability, which can be measured in vivo by urinary excretion after oral lactulose and mannitol ingestion. Inadvertent dietary consumption of (12) Carbon ((12) C, regular) mannitol in food or from other sources may interfere with the test's interpretation. (13) Carbon ((13) C) constitutes 1% of carbon in nature and (13) C mannitol is a stable isotope. Our aim was to determine the performance of (13) C mannitol for measurement of intestinal permeability. METHODS: Ten healthy volunteers underwent intestinal permeability assay using coadministered (12) C mannitol, (13) C mannitol and lactulose, followed by timed urine collections. Urinary sugar concentrations were measured using tandem high performance liquid chromatography-mass spectrometry. KEY RESULTS: We found that (13) C mannitol can be distinguishable from (12) C mannitol on tandem mass spectrometry. In addition, (13) C mannitol had ~20-fold lower baseline contamination compared to (12) C mannitol. We describe here the (13) C mannitol assay method for the measurement of intestinal permeability. CONCLUSIONS & INFERENCES: In conclusion, (13) C mannitol is superior to (12) C mannitol for measurement of intestinal permeability. It avoids issues with baseline contamination and erratic excretions during the testing period.
Authors: Archana S Rao; Michael Camilleri; Deborah J Eckert; Irene Busciglio; Duane D Burton; Michael Ryks; Banny S Wong; Jesse Lamsam; Ravinder Singh; Alan R Zinsmeister Journal: Am J Physiol Gastrointest Liver Physiol Date: 2011-08-11 Impact factor: 4.052
Authors: Hannah E Smith; Kelsey N Ryan; Kevin B Stephenson; Claire Westcott; Chrissie Thakwalakwa; Ken Maleta; Jacqueline Y Cheng; J Thomas Brenna; Robert J Shulman; Indi Trehan; Mark J Manary Journal: J Nutr Date: 2014-10-01 Impact factor: 4.798
Authors: C P Kelly; P H R Green; J A Murray; A Dimarino; A Colatrella; D A Leffler; T Alexander; R Arsenescu; F Leon; J G Jiang; L A Arterburn; B M Paterson; R N Fedorak Journal: Aliment Pharmacol Ther Date: 2012-11-19 Impact factor: 8.171
Authors: Paweł Kubica; Agata Kot-Wasik; Andrzej Wasik; Jacek Namieśnik; Piotr Landowski Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2012-09-04 Impact factor: 3.205
Authors: M Camilleri; K Madsen; R Spiller; B Greenwood-Van Meerveld; B G Van Meerveld; G N Verne Journal: Neurogastroenterol Motil Date: 2012-06 Impact factor: 3.598
Authors: Shoko Edogawa; Adam L Edwinson; Stephanie A Peters; Lakshmikanth L Chikkamenahalli; Wendy Sundt; Sara Graves; Sakteesh V Gurunathan; Margaret Breen-Lyles; Stephen Johnson; Roy Dyer; Rondell Graham; Jun Chen; Purna Kashyap; Gianrico Farrugia; Madhusudan Grover Journal: Gut Date: 2019-03-28 Impact factor: 23.059
Authors: Stephanie A Peters; Shoko Edogawa; Wendy J Sundt; Roy B Dyer; Daniel A Dalenberg; Amelia Mazzone; Ravinder J Singh; Natalie Moses; Thomas C Smyrk; Christopher Weber; David R Linden; Wallace K MacNaughton; Jerrold R Turner; Michael Camilleri; David A Katzka; Gianrico Farrugia; Madhusudan Grover Journal: Am J Gastroenterol Date: 2017-03-21 Impact factor: 10.864
Authors: Shoko Edogawa; Stephanie A Peters; Gregory D Jenkins; Sakteesh V Gurunathan; Wendy J Sundt; Stephen Johnson; Ryan J Lennon; Roy B Dyer; Michael Camilleri; Purna C Kashyap; Gianrico Farrugia; Jun Chen; Ravinder J Singh; Madhusudan Grover Journal: FASEB J Date: 2018-06-13 Impact factor: 5.191
Authors: Piero Portincasa; Leonilde Bonfrate; Mohamad Khalil; Maria De Angelis; Francesco Maria Calabrese; Mauro D'Amato; David Q-H Wang; Agostino Di Ciaula Journal: Biomedicines Date: 2021-12-31