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Literature DB >> 2691360

Characterization of phospholipase A2 of mycoplasma species.

Abstract

As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The purified enzyme was found to be heat-labile, active at alkaline pH, revealing a single band in polyacrylamide gel electrophoresis. Metal ions such as calcium and barium, increased its activity whereas solvents at high concentrations decreased it. It was resistant to surfactants.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1989 PMID： 2691360 DOI： 10.1007/bf02814471

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

27 in total

1. Phosphorus assay in column chromatography.

Authors: G R BARTLETT
Journal: J Biol Chem Date: 1959-03 Impact factor: 5.157

2. The estimation of phospholipase A activity in aqueous systems.

Authors: W L MAGEE; R H THOMPSON
Journal: Biochem J Date: 1960-12 Impact factor: 3.857

3. Organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases.

Authors: R F Zwaal; B Roelofsen; P Comfurius; L L van Deenen
Journal: Biochim Biophys Acta Date: 1975-09-16

4. A phospholipase specific for sphingomyelin from Clostridium perfringens.

Authors: I Pastan; V Macchia; R Katzen
Journal: J Biol Chem Date: 1968-07-10 Impact factor: 5.157

5. Production of enzymes and toxins by hospital strains of Pseudomonas aeruginosa in relation to serotype and phage-typing pattern.

Authors: B Wretlind; L Hedén; L Sjöberg; T Wadström
Journal: J Med Microbiol Date: 1973-02 Impact factor: 2.472

Characterization of phospholipase A2 of mycoplasma species.

1. Phosphorus assay in column chromatography.

2. The estimation of phospholipase A activity in aqueous systems.

3. Organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases.

4. A phospholipase specific for sphingomyelin from Clostridium perfringens.

5. Production of enzymes and toxins by hospital strains of Pseudomonas aeruginosa in relation to serotype and phage-typing pattern.

6. Direct haemolytic activity of phospholipase A.

7. Studies on the phospholipase A in larvae of Culex pipiens fatigans.

8. Phospholipase A-production in Staphylococcus aureus.

9. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism.

10. Placental infection with Mycoplasma homonis and Ureaplasma urealyticum: clinical correlation.

1. Purification and kinetics of extracellular phospholipase A of Salmonella newport.

2. Characterization of phospholipase A1, A2, C activity in Ureaplasma urealyticum membranes.

3. Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity.

4. Localization of endogenous activity of phospholipases A and C in Ureaplasma urealyticum.