Charalampos Siristatidis1, Paraskevi Vogiatzi2, Christos Varounis3, Marily Askoxylaki2, Charalampos Chrelias4, Nikolaos Papantoniou4. 1. Assisted Reproduction Unit, Third Department of Obstetrics and Gynecology, Athens University School of Medicine, Attikon Hospital, Athens, Greece harrysiri@yahoo.gr. 2. Assisted Reproduction Unit, Third Department of Obstetrics and Gynecology, Athens University School of Medicine, Attikon Hospital, Athens, Greece. 3. Second Department of Cardiology, Athens University School of Medicine, Attikon Hospital, Athens, Greece. 4. Third Department of Obstetrics and Gynecology, Athens University School of Medicine, Attikon Hospital, Athens, Greece.
Abstract
UNLABELLED: BACKROUND/AIM: Reactive oxygen species (ROS) are involved in critical biological processes in human reproduction. The aim of this study was to evaluate the association of embryo quality following in vitro fertilization (IVF), with ROS levels in the serum and follicular fluid (FF). MATERIALS AND METHODS: Eighty-five participants underwent ovarian stimulation and IVF; ROS levels were measured in blood samples on the day of oocyte retrieval and in the FF from follicular aspirates using enzyme-linked immunosorbent assay. These values were associated with the quality of embryos generated. RESULTS: Univariable zero-inflated Poisson model revealed that ROS levels at both oocyte retrieval and in FF were not associated with the number of grade I, II, III and IV embryos (p>0.05). Age, body mass index, stimulation protocol and smoking status were not associated with the number of embryos of any grade (p>0.05). CONCLUSION: Neither ROS levels in serum nor in FF are associated with the quality of embryos produced following IVF.
UNLABELLED: BACKROUND/AIM: Reactive oxygen species (ROS) are involved in critical biological processes in human reproduction. The aim of this study was to evaluate the association of embryo quality following in vitro fertilization (IVF), with ROS levels in the serum and follicular fluid (FF). MATERIALS AND METHODS: Eighty-five participants underwent ovarian stimulation and IVF; ROS levels were measured in blood samples on the day of oocyte retrieval and in the FF from follicular aspirates using enzyme-linked immunosorbent assay. These values were associated with the quality of embryos generated. RESULTS: Univariable zero-inflated Poisson model revealed that ROS levels at both oocyte retrieval and in FF were not associated with the number of grade I, II, III and IV embryos (p>0.05). Age, body mass index, stimulation protocol and smoking status were not associated with the number of embryos of any grade (p>0.05). CONCLUSION: Neither ROS levels in serum nor in FF are associated with the quality of embryos produced following IVF.
Authors: Katarzyna Olszak-Wąsik; Anna Bednarska-Czerwińska; Anita Olejek; Andrzej Tukiendorf Journal: Oxid Med Cell Longev Date: 2019-11-26 Impact factor: 6.543