Literature DB >> 26912366

Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak.

Zachary A Bornholdt1, Hannah L Turner2, Charles D Murin3, Wen Li4, Devin Sok1, Colby A Souders5, Ashley E Piper6, Arthur Goff6, Joshua D Shamblin6, Suzanne E Wollen6, Thomas R Sprague6, Marnie L Fusco1, Kathleen B J Pommert1, Lisa A Cavacini5, Heidi L Smith5, Mark Klempner5, Keith A Reimann5, Eric Krauland4, Tillman U Gerngross4, Karl D Wittrup4, Erica Ollmann Saphire1, Dennis R Burton7, Pamela J Glass6, Andrew B Ward2, Laura M Walker4.   

Abstract

Antibodies targeting the Ebola virus surface glycoprotein (EBOV GP) are implicated in protection against lethal disease, but the characteristics of the human antibody response to EBOV GP remain poorly understood. We isolated and characterized 349 GP-specific monoclonal antibodies (mAbs) from the peripheral B cells of a convalescent donor who survived the 2014 EBOV Zaire outbreak. Remarkably, 77% of the mAbs neutralize live EBOV, and several mAbs exhibit unprecedented potency. Structures of selected mAbs in complex with GP reveal a site of vulnerability located in the GP stalk region proximal to the viral membrane. Neutralizing antibodies targeting this site show potent therapeutic efficacy against lethal EBOV challenge in mice. The results provide a framework for the design of new EBOV vaccine candidates and immunotherapies.
Copyright © 2016, American Association for the Advancement of Science.

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Year:  2016        PMID： 26912366      PMCID： PMC4900763          DOI： 10.1126/science.aad5788

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  39 in total

1.  Crystal structure of the Ebola virus membrane fusion subunit, GP2, from the envelope glycoprotein ectodomain.

Authors:  W Weissenhorn; A Carfí; K H Lee; J J Skehel; D C Wiley
Journal:  Mol Cell       Date:  1998-11       Impact factor: 17.970

2.  Divergent human populations show extensive shared IGK rearrangements in peripheral blood B cells.

Authors:  Katherine Jean Louise Jackson; Yan Wang; Bruno A Gaeta; William Pomat; Peter Siba; Janet Rimmer; William A Sewell; Andrew M Collins
Journal:  Immunogenetics       Date:  2011-07-26       Impact factor: 2.846

3.  Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals.

Authors:  Johannes F Scheid; Hugo Mouquet; Niklas Feldhahn; Michael S Seaman; Klara Velinzon; John Pietzsch; Rene G Ott; Robert M Anthony; Henry Zebroski; Arlene Hurley; Adhuna Phogat; Bimal Chakrabarti; Yuxing Li; Mark Connors; Florencia Pereyra; Bruce D Walker; Hedda Wardemann; David Ho; Richard T Wyatt; John R Mascola; Jeffrey V Ravetch; Michel C Nussenzweig
Journal:  Nature       Date:  2009-03-15       Impact factor: 49.962

4.  Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques.

Authors:  Gene Garrard Olinger; James Pettitt; Do Kim; Cara Working; Ognian Bohorov; Barry Bratcher; Ernie Hiatt; Steven D Hume; Ashley K Johnson; Josh Morton; Michael Pauly; Kevin J Whaley; Calli M Lear; Julia E Biggins; Corinne Scully; Lisa Hensley; Larry Zeitlin
Journal:  Proc Natl Acad Sci U S A       Date:  2012-10-15       Impact factor: 11.205

5.  Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor.

Authors:  Jeffrey E Lee; Marnie L Fusco; Ann J Hessell; Wendelien B Oswald; Dennis R Burton; Erica Ollmann Saphire
Journal:  Nature       Date:  2008-07-10       Impact factor: 49.962

6.  Improved PEP-FOLD Approach for Peptide and Miniprotein Structure Prediction.

Authors:  Yimin Shen; Julien Maupetit; Philippe Derreumaux; Pierre Tufféry
Journal:  J Chem Theory Comput       Date:  2014-10-14       Impact factor: 6.006

7.  Individual variation in the germline Ig gene repertoire inferred from variable region gene rearrangements.

Authors:  Scott D Boyd; Bruno A Gaëta; Katherine J Jackson; Andrew Z Fire; Eleanor L Marshall; Jason D Merker; Jay M Maniar; Lyndon N Zhang; Bita Sahaf; Carol D Jones; Birgitte B Simen; Bozena Hanczaruk; Khoa D Nguyen; Kari C Nadeau; Michael Egholm; David B Miklos; James L Zehnder; Andrew M Collins
Journal:  J Immunol       Date:  2010-05-21       Impact factor: 5.422

8.  Antigenic subversion: a novel mechanism of host immune evasion by Ebola virus.

Authors:  Gopi S Mohan; Wenfang Li; Ling Ye; Richard W Compans; Chinglai Yang
Journal:  PLoS Pathog       Date:  2012-12-13       Impact factor: 6.823

9.  Serverification of molecular modeling applications: the Rosetta Online Server that Includes Everyone (ROSIE).

Authors:  Sergey Lyskov; Fang-Chieh Chou; Shane Ó Conchúir; Bryan S Der; Kevin Drew; Daisuke Kuroda; Jianqing Xu; Brian D Weitzner; P Douglas Renfrew; Parin Sripakdeevong; Benjamin Borgo; James J Havranek; Brian Kuhlman; Tanja Kortemme; Richard Bonneau; Jeffrey J Gray; Rhiju Das
Journal:  PLoS One       Date:  2013-05-22       Impact factor: 3.240

10.  Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp.

Authors:  Xiangguo Qiu; Gary Wong; Jonathan Audet; Alexander Bello; Lisa Fernando; Judie B Alimonti; Hugues Fausther-Bovendo; Haiyan Wei; Jenna Aviles; Ernie Hiatt; Ashley Johnson; Josh Morton; Kelsi Swope; Ognian Bohorov; Natasha Bohorova; Charles Goodman; Do Kim; Michael H Pauly; Jesus Velasco; James Pettitt; Gene G Olinger; Kevin Whaley; Bianli Xu; James E Strong; Larry Zeitlin; Gary P Kobinger
Journal:  Nature       Date:  2014-08-29       Impact factor: 49.962
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  112 in total

1.  A Two-Antibody Pan-Ebolavirus Cocktail Confers Broad Therapeutic Protection in Ferrets and Nonhuman Primates.

Authors:  Zachary A Bornholdt; Andrew S Herbert; Chad E Mire; Shihua He; Robert W Cross; Anna Z Wec; Dafna M Abelson; Joan B Geisbert; Rebekah M James; Md Niaz Rahim; Wenjun Zhu; Viktoriya Borisevich; Logan Banadyga; Bronwyn M Gunn; Krystle N Agans; Ariel S Wirchnianski; Eileen Goodwin; Kevin Tierney; William S Shestowsky; Ognian Bohorov; Natasha Bohorova; Jesus Velasco; Eric Ailor; Do Kim; Michael H Pauly; Kevin J Whaley; Galit Alter; Laura M Walker; Kartik Chandran; Larry Zeitlin; Xiangguo Qiu; Thomas W Geisbert; John M Dye
Journal:  Cell Host Microbe       Date:  2019-01-09       Impact factor: 21.023

2.  Development of a Human Antibody Cocktail that Deploys Multiple Functions to Confer Pan-Ebolavirus Protection.

Authors:  Anna Z Wec; Zachary A Bornholdt; Shihua He; Andrew S Herbert; Eileen Goodwin; Ariel S Wirchnianski; Bronwyn M Gunn; Zirui Zhang; Wenjun Zhu; Guodong Liu; Dafna M Abelson; Crystal L Moyer; Rohit K Jangra; Rebekah M James; Russell R Bakken; Natasha Bohorova; Ognian Bohorov; Do H Kim; Michael H Pauly; Jesus Velasco; Robert H Bortz; Kevin J Whaley; Tracey Goldstein; Simon J Anthony; Galit Alter; Laura M Walker; John M Dye; Larry Zeitlin; Xiangguo Qiu; Kartik Chandran
Journal:  Cell Host Microbe       Date:  2019-01-09       Impact factor: 21.023

Review 3.  Principles of Broad and Potent Antiviral Human Antibodies: Insights for Vaccine Design.

Authors:  James E Crowe
Journal:  Cell Host Microbe       Date:  2017-08-09       Impact factor: 21.023

4.  Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability.

Authors:  Xuelian Zhao; Katie A Howell; Shihua He; Jennifer M Brannan; Anna Z Wec; Edgar Davidson; Hannah L Turner; Chi-I Chiang; Lin Lei; J Maximilian Fels; Hong Vu; Sergey Shulenin; Ashley N Turonis; Ana I Kuehne; Guodong Liu; Mi Ta; Yimeng Wang; Christopher Sundling; Yongli Xiao; Jennifer S Spence; Benjamin J Doranz; Frederick W Holtsberg; Andrew B Ward; Kartik Chandran; John M Dye; Xiangguo Qiu; Yuxing Li; M Javad Aman
Journal:  Cell       Date:  2017-05-18       Impact factor: 41.582

5.  Antibodies from a Human Survivor Define Sites of Vulnerability for Broad Protection against Ebolaviruses.

Authors:  Anna Z Wec; Andrew S Herbert; Charles D Murin; Elisabeth K Nyakatura; Dafna M Abelson; J Maximilian Fels; Shihua He; Rebekah M James; Marc-Antoine de La Vega; Wenjun Zhu; Russell R Bakken; Eileen Goodwin; Hannah L Turner; Rohit K Jangra; Larry Zeitlin; Xiangguo Qiu; Jonathan R Lai; Laura M Walker; Andrew B Ward; John M Dye; Kartik Chandran; Zachary A Bornholdt
Journal:  Cell       Date:  2017-05-18       Impact factor: 41.582

Review 6.  Achieving cross-reactivity with pan-ebolavirus antibodies.

Authors:  Liam B King; Jacob C Milligan; Brandyn R West; Sharon L Schendel; Erica Ollmann Saphire
Journal:  Curr Opin Virol       Date:  2019-03-15       Impact factor: 7.090

7.  Lack of Evidence for a Substantial Rate of Templated Mutagenesis in B Cell Diversification.

Authors:  Julia Fukuyama; Branden J Olson; Frederick A Matsen
Journal:  J Immunol       Date:  2020-07-15       Impact factor: 5.422

8.  Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection.

Authors:  Erica Ollmann Saphire; Sharon L Schendel; Marnie L Fusco; Karthik Gangavarapu; Bronwyn M Gunn; Anna Z Wec; Peter J Halfmann; Jennifer M Brannan; Andrew S Herbert; Xiangguo Qiu; Kshitij Wagh; Shihua He; Elena E Giorgi; James Theiler; Kathleen B J Pommert; Tyler B Krause; Hannah L Turner; Charles D Murin; Jesper Pallesen; Edgar Davidson; Rafi Ahmed; M Javad Aman; Alexander Bukreyev; Dennis R Burton; James E Crowe; Carl W Davis; George Georgiou; Florian Krammer; Christos A Kyratsous; Jonathan R Lai; Cory Nykiforuk; Michael H Pauly; Pramila Rijal; Ayato Takada; Alain R Townsend; Viktor Volchkov; Laura M Walker; Cheng-I Wang; Larry Zeitlin; Benjamin J Doranz; Andrew B Ward; Bette Korber; Gary P Kobinger; Kristian G Andersen; Yoshihiro Kawaoka; Galit Alter; Kartik Chandran; John M Dye
Journal:  Cell       Date:  2018-08-09       Impact factor: 41.582

9.  Vaccine-Mediated Induction of an Ebolavirus Cross-Species Antibody Binding to Conserved Epitopes on the Glycoprotein Heptad Repeat 2/Membrane-Proximal External Junction.

Authors:  Alberto Cagigi; Aurélie Ploquin; Thomas Niezold; Yan Zhou; Yaroslav Tsybovsky; John Misasi; Nancy J Sullivan
Journal:  J Infect Dis       Date:  2018-11-22       Impact factor: 5.226

Review 10.  The structural basis for filovirus neutralization by monoclonal antibodies.

Authors:  Liam B King; Brandyn R West; Sharon L Schendel; Erica Ollmann Saphire
Journal:  Curr Opin Immunol       Date:  2018-06-22       Impact factor: 7.486
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