Shih-Chun Yang1,2, Ibrahim A Aljuffali3, Calvin T Sung4, Chwan-Fwu Lin5, Jia-You Fang1,6,7. 1. Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan. 2. Research Center for Industry of Human Ecology, Chang Gung University of Science & Technology, Kweishan, Taoyuan, Taiwan. 3. Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. 4. Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, USA. 5. Department of Cosmetic Science, Chang Gung University of Science & Technology, Kweishan, Taoyuan, Taiwan. 6. Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Kweishan, Taoyuan, Taiwan. 7. Immunology Consortium, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan.
Abstract
AIM: Here we evaluated the antibacterial efficacy of soyaethyl morpholinium ethosulfate (SME) micelles as an inherent bactericide against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). METHODOLOGY: The antimicrobial activity was examined by in vitro culture model and murine model of skin infection. Cationic micelles formed by benzalkonium chloride or cetylpyridinium chloride were used for comparison. RESULTS: The minimum inhibitory concentration and minimum bactericidal concentration against S. aureus and MRSA were 1.71-3.42 and 1.71-6.84 μg/ml, respectively. Topical administration of SME micelles significantly decreased the cutaneous infection and MRSA load in mice. The killing of bacteria was caused by direct cell wall/membrane rupture. SME micelles also penetrated into the bacteria to elicit a Fenton reaction and oxidative stress. CONCLUSION: SME micelles have potential as antimicrobial agents due to their lethal effect against S. aureus and MRSA with a low toxicity to mammalian cells.
AIM: Here we evaluated the antibacterial efficacy of soyaethyl morpholinium ethosulfate (SME) micelles as an inherent bactericide against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). METHODOLOGY: The antimicrobial activity was examined by in vitro culture model and murine model of skin infection. Cationic micelles formed by benzalkonium chloride or cetylpyridinium chloride were used for comparison. RESULTS: The minimum inhibitory concentration and minimum bactericidal concentration against S. aureus and MRSA were 1.71-3.42 and 1.71-6.84 μg/ml, respectively. Topical administration of SME micelles significantly decreased the cutaneous infection and MRSA load in mice. The killing of bacteria was caused by direct cell wall/membrane rupture. SME micelles also penetrated into the bacteria to elicit a Fenton reaction and oxidative stress. CONCLUSION:SME micelles have potential as antimicrobial agents due to their lethal effect against S. aureus and MRSA with a low toxicity to mammalian cells.
Entities:
Keywords:
antimicrobial; methicillin-resistant S. aureus; micelle; skin; soyaethyl morpholinium ethosulfate