Radka Gromnicova1, Canan Ugur Yilmaz2, Nurcan Orhan3, Mehmet Kaya4, Heather Davies1, Phil Williams5, Ignacio A Romero1, Basil Sharrack6, David Male1. 1. Department of Life, Health & Chemical Sciences, The Open University, Milton Keynes, MK7 6AA, UK. 2. Department of Laboratory Animal Science, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. 3. Department of Neuroscience, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. 4. Department of Physiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. 5. Midatech Pharma Plc, Abingdon, UK. 6. Department of Neurology, University of Sheffield, Sheffield, UK.
Abstract
AIM: To identify the localization of glucose-coated gold nanoparticles within cells of the brain after intravascular infusion which may point to the mechanism by which they cross the blood-brain barrier. MATERIALS & METHODS: Tissue distribution of the nanoparticles was measured by inductively-coupled-mass spectrometry and localization within the brain by histochemistry and electron microscopy. RESULTS & CONCLUSION: Nanoparticles were identified within neurons and glial cells more than 10 μm from the nearest microvessel within 10 min of intracarotid infusion. Their distribution indicated movement across the endothelial cytosol, and direct transfer between cells of the brain. The rapid movement of this class of nanoparticle (<5 nm) into the brain demonstrates their potential to carry therapeutic biomolecules or imaging reagents.
AIM: To identify the localization of glucose-coated gold nanoparticles within cells of the brain after intravascular infusion which may point to the mechanism by which they cross the blood-brain barrier. MATERIALS & METHODS: Tissue distribution of the nanoparticles was measured by inductively-coupled-mass spectrometry and localization within the brain by histochemistry and electron microscopy. RESULTS & CONCLUSION: Nanoparticles were identified within neurons and glial cells more than 10 μm from the nearest microvessel within 10 min of intracarotid infusion. Their distribution indicated movement across the endothelial cytosol, and direct transfer between cells of the brain. The rapid movement of this class of nanoparticle (<5 nm) into the brain demonstrates their potential to carry therapeutic biomolecules or imaging reagents.
Entities:
Keywords:
blood–brain barrier; brain; brain endothelium; cortex; drug delivery; glia; glyconanoparticles; gold nanoparticles; neurons; transmission electron microscopy
Authors: Radka Gromnicova; Mehmet Kaya; Ignacio A Romero; Phil Williams; Simon Satchell; Basil Sharrack; David Male Journal: PLoS One Date: 2016-08-25 Impact factor: 3.240
Authors: Kasper Bendix Johnsen; Martin Bak; Paul Joseph Kempen; Fredrik Melander; Annette Burkhart; Maj Schneider Thomsen; Morten Schallburg Nielsen; Torben Moos; Thomas Lars Andresen Journal: Theranostics Date: 2018-05-24 Impact factor: 11.556
Authors: Marcelle Silva-Abreu; Ana Cristina Calpena; Pol Andrés-Benito; Ester Aso; Ignacio A Romero; David Roig-Carles; Radka Gromnicova; Marta Espina; Isidre Ferrer; María Luisa García; David Male Journal: Int J Nanomedicine Date: 2018-09-20