| Literature DB >> 26910244 |
Swati Garg1, Kanjaksha Ghosh1, Manisha Madkaikar2.
Abstract
A putative leukemic stem cell population in AML defined by Lin-/CD38-/CD34+ is associated with disease prognosis. Antigens that are differentially expressed on normal and leukemic stem cells are known for their potential use in AML prognosis, but their differential expression on blast and pLSC has not been extensively explored in the clinical setting. In the present study, we sought to identify expression level differences of CD44, CD90, CD96, CD123, CD25 and intracellular WT-1; on AML blasts and Lin-/CD38-/CD34+ population in CD34+ AMLs using multi-parametric flow cytometry. Paired analysis of marker expression intensity ratio was done on pLSC and blast populations from 30 AML samples. We found no statistically significant difference in the expression levels of these markers between bulk blasts and carefully defined subpopulations. Our results suggest that expression of these antigens can be evaluated on AML blasts rather than pLSC for economic and informative reasons.Entities:
Keywords: Acute myeloid leukemia; CD44; CD96; Putative leukemic stem cells
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Year: 2016 PMID： 26910244 DOI： 10.1007/s12185-016-1961-y
Source DB: PubMed Journal: Int J Hematol ISSN： 0925-5710 Impact factor: 2.490