Yucheng Wang1, Ximing Wu2, Jia Chen3, Rehab Amin2, Min Lu2, Brijesh Bhayana2, Jie Zhao2, Clinton K Murray4, Michael R Hamblin5, David C Hooper6, Tianhong Dai2. 1. Department of Laser Medicine, Chinese PLA General Hospital, Beijing College of Medicine, Nankai University, Tianjin Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston. 2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston. 3. Shanghai Dermatology Hospital, China Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston. 4. Infectious Disease Service, Brooke Army Medical Center, Fort Sam Houston, Texas. 5. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts. 6. Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston.
Abstract
BACKGROUND: Biofilms affect >80% bacterial infections in human and are usually difficult to eradicate because of their inherent drug resistance. METHODS: We investigated the effectiveness of antimicrobial blue light (aBL) (wavelength, 415 nm) for inactivating Acinetobacter baumannii or Pseudomonas aeruginosa biofilms in 96-well microplates or infected mouse burn wounds. RESULTS: In vitro, in 96-well microplates, exposure of 24-hour-old and 72-hour-old A. baumannii biofilms to 432 J/cm(2) aBL resulted in inactivation of 3.59 log10 and 3.18 log10 colony-forming units (CFU), respectively. For P. aeruginosa biofilms, similar levels of inactivation-3.02 log10 and 3.12 log10 CFU, respectively-were achieved. In mouse burn wounds infected with 5 × 10(6) CFU ofA. baumannii, approximately 360 J/cm(2) and 540 J/cm(2) aBL was required to inactivate 3 log10 CFU in biofilms when delivered 24 and 48 hours, respectively, after bacterial inoculation. High-performance liquid chromatography analysis revealed the presence of endogenous porphyrins in both A. baumannii and P. aeruginosa TUNEL assay detected no apoptotic cells in aBL-irradiated mouse skin at up to 24 hours after aBL exposure (540 J/cm(2)). CONCLUSIONS: aBL has antimicrobial activity in biofilms ofA. baumannii and P. aeruginosa and is a potential therapeutic approach for biofilm-related infections.
BACKGROUND: Biofilms affect >80% bacterial infections in human and are usually difficult to eradicate because of their inherent drug resistance. METHODS: We investigated the effectiveness of antimicrobial blue light (aBL) (wavelength, 415 nm) for inactivating Acinetobacter baumannii or Pseudomonas aeruginosa biofilms in 96-well microplates or infected mouse burn wounds. RESULTS: In vitro, in 96-well microplates, exposure of 24-hour-old and 72-hour-old A. baumannii biofilms to 432 J/cm(2) aBL resulted in inactivation of 3.59 log10 and 3.18 log10 colony-forming units (CFU), respectively. For P. aeruginosa biofilms, similar levels of inactivation-3.02 log10 and 3.12 log10 CFU, respectively-were achieved. In mouse burn wounds infected with 5 × 10(6) CFU ofA. baumannii, approximately 360 J/cm(2) and 540 J/cm(2) aBL was required to inactivate 3 log10 CFU in biofilms when delivered 24 and 48 hours, respectively, after bacterial inoculation. High-performance liquid chromatography analysis revealed the presence of endogenous porphyrins in both A. baumannii and P. aeruginosa TUNEL assay detected no apoptotic cells in aBL-irradiated mouse skin at up to 24 hours after aBL exposure (540 J/cm(2)). CONCLUSIONS:aBL has antimicrobial activity in biofilms ofA. baumannii and P. aeruginosa and is a potential therapeutic approach for biofilm-related infections.
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