Huijuan Wang1, Min He2, Yunhua Hou3, Saiying Chen4, Xiaojie Zhang5, Mingshun Zhang6, Xiaohui Ji7. 1. State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, China; Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: wanghuijuan@njmu.edu.cn. 2. Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: hemin.820@163.com. 3. Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. 4. Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: csydd0324@126.com. 5. Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: njzhangxj@163.com. 6. Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: mingshunzhang@njmu.edu.cn. 7. State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, China; Department of Immunology, Nanjing Medical University, Nanjing, 210029, China. Electronic address: jixiaohui@njmu.edu.cn.
Abstract
OBJECTIVE: Decidual macrophages (dMΦs) have been implicated in fetal tolerance, but little information is known regarding their differentiation capacity and interactions with T cells. The present study aimed to investigate the immunological characteristics of dMΦs at mid and term pregnancy. METHODS: The dMΦs were analyzed for their phenotypes and cytokine production by flow cytometry and ELISA, respectively. The transendothelial trafficking model was implemented to allow the dMΦs to differentiate. The differentiated cells from dMΦs were also measured for their phenotypes and cytokine production with same methods. The capacity of dMΦs or the differentiated cells from dMΦs to stimulate allogeneic T lymphocyte proliferation was evaluated by T lymphocyte stimulation assays. T cell differentiation was determined by flow cytometry. RESULTS: The dMΦs in the mid-pregnancy (Mid-dMΦs) resembled the M2 phenotype. The differentiated cells from Mid-dMΦs had little stimulatory capacity on T cell proliferation and favored regulatory T cell differentiation. The dMΦs at term differentiated into dendritic (DC)-like cells, stimulating T cell activation, proliferation, and differentiation into IFN-γ-producing T cellsdecidual CONCLUSIONS: The present study suggests that the differences in phenotypes and cytokine production between Mid- and Term-dMΦs relate to their different roles in the homeostasis of the maternal-fetal interface. Mid-dMΦs differentiate into DC-like cells with immunosuppressive properties, playing an important role in maintaining homeostasis required for a successful pregnancy. Term-dMΦs differentiate into DC-like cells with immunostimulatory properties, likely involved in the activation of labor. The different differentiation capacities of dMΦs in the varied pregnancy stages may be due to the placental microenvironment.
OBJECTIVE: Decidual macrophages (dMΦs) have been implicated in fetal tolerance, but little information is known regarding their differentiation capacity and interactions with T cells. The present study aimed to investigate the immunological characteristics of dMΦs at mid and term pregnancy. METHODS: The dMΦs were analyzed for their phenotypes and cytokine production by flow cytometry and ELISA, respectively. The transendothelial trafficking model was implemented to allow the dMΦs to differentiate. The differentiated cells from dMΦs were also measured for their phenotypes and cytokine production with same methods. The capacity of dMΦs or the differentiated cells from dMΦs to stimulate allogeneic T lymphocyte proliferation was evaluated by T lymphocyte stimulation assays. T cell differentiation was determined by flow cytometry. RESULTS: The dMΦs in the mid-pregnancy (Mid-dMΦs) resembled the M2 phenotype. The differentiated cells from Mid-dMΦs had little stimulatory capacity on T cell proliferation and favored regulatory T cell differentiation. The dMΦs at term differentiated into dendritic (DC)-like cells, stimulating T cell activation, proliferation, and differentiation into IFN-γ-producing T cellsdecidual CONCLUSIONS: The present study suggests that the differences in phenotypes and cytokine production between Mid- and Term-dMΦs relate to their different roles in the homeostasis of the maternal-fetal interface. Mid-dMΦs differentiate into DC-like cells with immunosuppressive properties, playing an important role in maintaining homeostasis required for a successful pregnancy. Term-dMΦs differentiate into DC-like cells with immunostimulatory properties, likely involved in the activation of labor. The different differentiation capacities of dMΦs in the varied pregnancy stages may be due to the placental microenvironment.
Authors: Xuezi Tian; Kaveri T S Aiyer; Johanna M Kapsenberg; Dave L Roelen; Marie-Louise van der Hoorn; Michael Eikmans Journal: Am J Reprod Immunol Date: 2021-12-04 Impact factor: 3.777
Authors: Syed F Hassnain Waqas; Anna Noble; Anh C Hoang; Grace Ampem; Manuela Popp; Sarah Strauß; Matthew Guille; Tamás Röszer Journal: J Leukoc Biol Date: 2017-06-22 Impact factor: 4.962