Yueling Jin1, Zhensheng Dai2. 1. Department of Pathology, Shanghai University of Medicine and Health Sciences, Shanghai 200237, China. 2. Department of Hematology, Shanghai Pudong Hospital Affiliated to Fudan University, Shanghai 201399, China. Electronic address: zhenshengdai@126.com.
Abstract
OBJECTIVE: This study aimed to explore the influence of USO1 on multiple myeloma (MM) cell proliferation and apoptosis and the related molecular mechanism. METHODS: The expression of USO1 and MIF in MM tissues and cells, normal bone marrow tissues and cells were determined by qRT-PCR and western blot assay. The cell proliferation and apoptosis of MM cells before and after knockdown of USO1 were determined by MTT assay and flow cytometry, respectively. Before and after knockdown of USO1, the expression of the proliferation-related genes cyclin D1, Mcm2 and PCNA in MM cells was determined by qRT-PCR and western blot assay. The protein level of p-Erk1/2 and MIF was determined by western blot assay and ELISA, respectively. RESULTS: The expression levels of USO1 and MIF in MM tissues and cells were much higher than those in normal bone marrow tissues and cells. Knockdown of USO1 resulted in the inhibited ability of cell proliferation and induced cell apoptosis. The expression of cyclin D1, Mcm2, PCNA and p-Erk1/2 decreased significantly after knockdown of USO1 as well as the decreased MIF secretion. CONCLUSION: USO1 gene may be a promising target for the therapy of human MM and its diagnosis marker.
OBJECTIVE: This study aimed to explore the influence of USO1 on multiple myeloma (MM) cell proliferation and apoptosis and the related molecular mechanism. METHODS: The expression of USO1 and MIF in MM tissues and cells, normal bone marrow tissues and cells were determined by qRT-PCR and western blot assay. The cell proliferation and apoptosis of MM cells before and after knockdown of USO1 were determined by MTT assay and flow cytometry, respectively. Before and after knockdown of USO1, the expression of the proliferation-related genes cyclin D1, Mcm2 and PCNA in MM cells was determined by qRT-PCR and western blot assay. The protein level of p-Erk1/2 and MIF was determined by western blot assay and ELISA, respectively. RESULTS: The expression levels of USO1 and MIF in MM tissues and cells were much higher than those in normal bone marrow tissues and cells. Knockdown of USO1 resulted in the inhibited ability of cell proliferation and induced cell apoptosis. The expression of cyclin D1, Mcm2, PCNA and p-Erk1/2 decreased significantly after knockdown of USO1 as well as the decreased MIF secretion. CONCLUSION:USO1 gene may be a promising target for the therapy of human MM and its diagnosis marker.
Authors: Anna Keogh; Lisa Ryan; Mutaz M Nur; Anne-Marie Baird; Siobhan Nicholson; Sinéad Cuffe; Gerard J Fitzmaurice; Ronan Ryan; Vincent K Young; Stephen P Finn; Steven G Gray Journal: Transl Lung Cancer Res Date: 2022-09
Authors: Amit Kumar Jaiswal; Hellen Truong; Tiffany M Tran; Tasha L Lin; David Casero; Michael O Alberti; Dinesh S Rao Journal: Sci Rep Date: 2021-06-23 Impact factor: 4.379