Literature DB >> 26894584

Correction: Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae.

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Abstract

Entities:  

Year:  2016        PMID： 26894584      PMCID： PMC4760700          DOI： 10.1371/journal.pone.0150127

Source DB:  PubMed          Journal:  PLoS One        ISSN： 1932-6203            Impact factor:   3.240


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There are errors in the Results, Table 2 and Table 3, which were introduced during the typesetting process. The publisher apologizes for these errors.
Table 2

Entry clones created in this study.

PlasmidPromoter
  pYS1S. cerevisiae CUP1
pYS2S. pombe ADH1
pYS3S. cerevisiae TEF
pYS6TetO7 -CYC1TATA
pYS7TetO2 -CYC1TATA
PlasmidORF
  pCG32yEGFP
pDHM7yEGFP-Cln2PEST
pCG55yEVenus
pYS61yEVenus-NLS*1
pCG98yEVenus-Cln2PEST-NLS
pCG40mCherry
pYS60mCherry-NLS
pYS19tTA
pYS20rtTA
pYS58AIDtTA
pYS57AIDrtTA
pCG72OsTIR1-9Myc

Promoter Entry clones were created with pDONR221P5-P2 and ORF Entry clones with pDONR221P1-P5r.

*1 Significant cytoplasmic fluorescence was observed when overexpressed, for example, by TEF promoter.

Table 3

Expression plasmid vectors constructed by Gateway recombination method in this study.

PlasmidPromoterORFMarkerDestination vector
  pCG52S. cerevisiaeTEFmCherryLEU2pDEST415TEFt7
pCG109S. pombe ADH1mCherry-NLSTRP1pDEST414TEFt7
pCG57S. cerevisiae TEFyEVenusLEU2pDEST415TEFt7
pRN1S. pombe ADH1yEVenus-NLSLEU2pDEST415TEFt7
pDHM57S. pombe ADH1yEVenus-Cln2PEST-NLSLEU2pDEST415TEFt7
pCM25S. cerevisiae CUP1yEVenus-Cln2PEST-NLSLEU2pDEST415TEFt7
pCG87TetO7-CYC1TATAmCherry-NLSTRP1pDEST414TEFt7
pCG103TetO7-CYC1TATAyEVenus-Cln2PEST-NLSTRP1pDEST414TEFt7
pCM20TetO7-CYC1TATAyEVenus-Cln2PEST-NLSLEU2pDEST415TEFt7
pCG84S. pombe ADH1tTAHIS3pDEST413TEFt7
pCG85S. pombe ADH1rtTAHIS3pDEST413TEFt7
pDHM19S. pombe ADH1AIDtTAHIS3pDEST413TEFt7
pDHM20S. pombe ADH1AIDrtTAHIS3pDEST413TEFt7
pCG112*1S. cerevisiae TEFtTAHIS3pDEST413TEFt7
pCG113S. cerevisiae TEFrtTAHIS3pDEST413TEFt7
pCG106*1S. cerevisiae TEFAIDtTAHIS3pDEST413TEFt7
pCG107S. cerevisiae TEFAIDrtTAHIS3pDEST413TEFt7
pMM6*2S. cerevisiaeTEFAIDrtTAURA3, MET15pDEST375
pCG81S. pombe ADH1OsTIR1-9MycURA3pDEST416TEFt7

*1 Yeast cells with these expression vectors showed poor growth with an unknown reason.

*2 Integration vector.

Promoter Entry clones were created with pDONR221P5-P2 and ORF Entry clones with pDONR221P1-P5r. *1 Significant cytoplasmic fluorescence was observed when overexpressed, for example, by TEF promoter. *1 Yeast cells with these expression vectors showed poor growth with an unknown reason. *2 Integration vector. There is an error in the penultimate sentence of the third paragraph of the “Construction of Yeast Gateway Vectors for One-step Gene Assembly” subsection of the Results. The correct sentence is: We compared the fluorescence produced by yEVenus-Cln2PEST-NLS and yEVenus-NLS when expressed by the constitutive promoter ADH1 (Fig 4B, C). There is an error in Table 2, “Entry clones created in this study.” Please see the corrected Table 2 here. There is an error in Table 3, “Expression plasmid vectors constructed by Gateway recombination method in this study.” Please see the corrected Table 3 here.
  1 in total

1.  Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae.

Authors:  Claudiu V Giuraniuc; Murray MacPherson; Yasushi Saka
Journal:  PLoS One       Date:  2013-05-10       Impact factor: 3.240
  1 in total

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