| Literature DB >> 2689221 |
M Ryd1, H Alfredsson, L Blomberg, A Andersson, A A Lindberg.
Abstract
A simple and rapid method for Shiga toxin purification based on specific binding to the Gal alpha 1----4Gal beta 1----4Glc globotrioside trisaccharide covalently linked to polyacryl/polyvinyl (Fractogel) has been developed. A cell-free sonicate-filtrate of Shigella dysenteriae type 1, strain 114Sd was passed over the globotrioside-Fractogel column, and bound toxin eluted with 6 M guanidine-HCl. A yield of 36 mg pure toxin/1 sonicate-filtrate was obtained, i.e. a one step 1224-fold purification. The recovery of biologically active toxin was 87%. The toxin was purified to homogeneity as shown by native PAGE and SDS-PAGE.Entities:
Mesh：
Substances：
Year: 1989 PMID： 2689221 DOI： 10.1016/0014-5793(89)81684-9
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124