Lili Du1, Runxiao Lv2, Xiaoyi Yang3, Shaohang Cheng4, Tingxian Ma5, Jing Xu4. 1. Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110122, People's Republic of China. Electronic address: dllbinglishengli@126.com. 2. Department of Spinal Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China. 3. Seven-year System, Department of Clinical Medicine, China Medical University, Shenyang 110122, People's Republic of China. 4. Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China. 5. Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110122, People's Republic of China.
Abstract
AIMS: Scar formation after wound repair affects people's daily life. Mesenchymal stem cells were reported to have a beneficial role in attenuating the scar formation. In the present study, placenta-derived mesenchymal stem cells (PMSCs) were isolated and the effects of hypoxic conditioned medium of PMSCs on scar formation were explored. MAIN METHODS: To evaluate the effect of hypoxia on PMSCs, proliferation of PMSCs was detected by trypan blue staining and the HIF-1α level was detected by western blot. Then in vivo scar formation assay was performed and the histopathologic changes were evaluated by HE staining and levels of TGF-β1 and collagen I were detected by quantitative real-time PCR. The IL-10 level was detected by ELISA and then migration of HFF-1 cells was detected by wound healing assay after treatment with IL-10 or IL-10 antibody. KEY FINDINGS: Our study showed that hypoxic conditioned medium of PMSCs reduced scar formation in vivo and inhibited the proliferation and migration of skin fibroblasts in vitro. Further mechanism study showed that, the level of IL-10 was affected by hypoxia, treatment with IL-10 mimicked the function of hypoxic conditioned medium of PMSCs and inhibition of IL-10 reversed the protective role of hypoxic conditioned medium of PMSCs. Thus, hypoxic conditioned medium of PMSCs may perform the protective role against scar formation through IL-10. SIGNIFICANCE: Our study reveals a possible mechanism of the protective effect of PMSCs against scar formation and provides evidence for the hypothesis that PMSCs may be a promising therapy for the treatment of wounds.
AIMS: Scar formation after wound repair affects people's daily life. Mesenchymal stem cells were reported to have a beneficial role in attenuating the scar formation. In the present study, placenta-derived mesenchymal stem cells (PMSCs) were isolated and the effects of hypoxic conditioned medium of PMSCs on scar formation were explored. MAIN METHODS: To evaluate the effect of hypoxia on PMSCs, proliferation of PMSCs was detected by trypan blue staining and the HIF-1α level was detected by western blot. Then in vivo scar formation assay was performed and the histopathologic changes were evaluated by HE staining and levels of TGF-β1 and collagen I were detected by quantitative real-time PCR. The IL-10 level was detected by ELISA and then migration of HFF-1 cells was detected by wound healing assay after treatment with IL-10 or IL-10 antibody. KEY FINDINGS: Our study showed that hypoxic conditioned medium of PMSCs reduced scar formation in vivo and inhibited the proliferation and migration of skin fibroblasts in vitro. Further mechanism study showed that, the level of IL-10 was affected by hypoxia, treatment with IL-10 mimicked the function of hypoxic conditioned medium of PMSCs and inhibition of IL-10 reversed the protective role of hypoxic conditioned medium of PMSCs. Thus, hypoxic conditioned medium of PMSCs may perform the protective role against scar formation through IL-10. SIGNIFICANCE: Our study reveals a possible mechanism of the protective effect of PMSCs against scar formation and provides evidence for the hypothesis that PMSCs may be a promising therapy for the treatment of wounds.
Authors: Michael S Hu; Mimi R Borrelli; H Peter Lorenz; Michael T Longaker; Derrick C Wan Journal: Stem Cells Int Date: 2018-05-20 Impact factor: 5.443
Authors: Ji Hyun Kim; Denethia S Green; Young Min Ju; Mollie Harrison; J William Vaughan; Anthony Atala; Sang Jin Lee; John D Jackson; Cory Nykiforuk; James J Yoo Journal: Front Bioeng Biotechnol Date: 2022-07-22