AIM: Development of EGF-liposomes (LP-EGF) for selective molecules delivery in tumors expressing EGFR. MATERIAL & METHODS: In vitro cellular interaction of EGF-LP and nontargeted liposomes (LP-N) was assayed at 37 and 4 °C in cells expressing different EGFR levels. Receptor-mediated uptake was investigated by competition with a monoclonal antibody anti-EGFR. Selective intracellular drug delivery and efficacy was tested by oxaliplatin encapsulation. In vivo biodistribution of LP-N and LP-EGF was done in xenograft model. RESULTS: LP-EGF was internalized by an active and selective mechanism through EGFR without receptor activation. Oxaliplatin LP-EGF decreased IC50 between 48 and 13% in cell EGFR+. LP-EGF was accumulated in tumor over 72 h postdosing, while LP-N in spleen. CONCLUSION: LP-EGF represents an attractive nanosystem for cancer therapy or diagnosis.
AIM: Development of EGF-liposomes (LP-EGF) for selective molecules delivery in tumors expressing EGFR. MATERIAL & METHODS: In vitro cellular interaction of EGF-LP and nontargeted liposomes (LP-N) was assayed at 37 and 4 °C in cells expressing different EGFR levels. Receptor-mediated uptake was investigated by competition with a monoclonal antibody anti-EGFR. Selective intracellular drug delivery and efficacy was tested by oxaliplatin encapsulation. In vivo biodistribution of LP-N and LP-EGF was done in xenograft model. RESULTS:LP-EGF was internalized by an active and selective mechanism through EGFR without receptor activation. OxaliplatinLP-EGF decreased IC50 between 48 and 13% in cell EGFR+. LP-EGF was accumulated in tumor over 72 h postdosing, while LP-N in spleen. CONCLUSION:LP-EGF represents an attractive nanosystem for cancer therapy or diagnosis.
Authors: Eloise Thomas; Jyothi U Menon; Joshua Owen; Irini Skaripa-Koukelli; Sheena Wallington; Michael Gray; Christophoros Mannaris; Veerle Kersemans; Danny Allen; Paul Kinchesh; Sean Smart; Robert Carlisle; Katherine A Vallis Journal: Theranostics Date: 2019-07-28 Impact factor: 11.600
Authors: Marina Martín-Contreras; Saúl A Navarro-Marchal; José Manuel Peula-García; Ana Belén Jódar-Reyes Journal: Pharmaceutics Date: 2022-02-10 Impact factor: 6.321
Authors: Kiran Kumar Solingapuram Sai; Xiaofei Chen; Zhe Li; Caigang Zhu; Kirtikar Shukla; Tom E Forshaw; Hanzhi Wu; Stephen A Vance; Buddhika Liyana Pathirannahel; Megan Madonna; Mark W Dewhirst; Allen W Tsang; Leslie B Poole; Nimmi Ramanujam; S Bruce King; Cristina M Furdui Journal: Redox Biol Date: 2021-12-18 Impact factor: 11.799