C J Nile1, D A Apatzidou2, R A Awang1,3, M P Riggio1, D F Kinane4, D F Lappin5. 1. Infection and Immunity Research Group, College of Medical, Veterinary and Life Sciences, University of Glasgow Dental School, 378 Sauchiehall Street, Glasgow, G2 3JZ, UK. 2. School of Dentistry, Aristotle University, Thessaloniki, Greece. 3. School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. 4. School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA. 5. Infection and Immunity Research Group, College of Medical, Veterinary and Life Sciences, University of Glasgow Dental School, 378 Sauchiehall Street, Glasgow, G2 3JZ, UK. david.lappin@glasgow.ac.uk.
Abstract
OBJECTIVES: The serum IL-17A:IL-17E ratio has previously been demonstrated to be a clinical marker of periodontitis. The aim of this study was to determine the effects of non-surgical periodontal treatment on the serum IL-17A:IL-17E ratio. MATERIALS AND METHODS: Forty chronic periodontitis patients completed this study and received periodontal treatment comprising scaling and root planing plus ultrasonic debridement. Clinical data were recorded at baseline, 6 weeks (R1) after treatment completion (full-mouth or quadrant-scaling and root planing) and 25 weeks after baseline (R2). Serum samples were taken at each time point and cytokines concentrations determined by ELISA. RESULTS: Following treatment, statistically significant reductions were noted in clinical parameters. However, IL-17A and IL-17E concentrations were significantly greater than baseline values before- and after-adjusting for smoking. The IL-17A:IL-17E ratio was lower at R1 and R2. Serum IL-6 and TNF levels were significantly lower at R1 only. Also exclusively at R1, serum IL-17A and IL-17E correlated positively with clinical parameters, while the IL-17A:IL-17E ratio correlated negatively with probing pocket depth and clinical attachment. CONCLUSION: Increased serum IL-17E and a reduced IL-17A:IL-17E ratio may be indicative and/or a consequence of periodontal therapy. Therefore, the role of IL-17E in periodontal disease progression and the healing process is worthy of further investigation. CLINICAL RELEVANCE: IL-17E may be a valuable biomarker to monitor the healing process following periodontal treatment as increased IL-17E levels and a reduced IL-17A:IL-17E ratio could reflect clinical improvements post-therapy. Therefore, monitoring serum IL-17E might be useful to identify individuals who require additional periodontal treatment.
OBJECTIVES: The serum IL-17A:IL-17E ratio has previously been demonstrated to be a clinical marker of periodontitis. The aim of this study was to determine the effects of non-surgical periodontal treatment on the serum IL-17A:IL-17E ratio. MATERIALS AND METHODS: Forty chronic periodontitispatients completed this study and received periodontal treatment comprising scaling and root planing plus ultrasonic debridement. Clinical data were recorded at baseline, 6 weeks (R1) after treatment completion (full-mouth or quadrant-scaling and root planing) and 25 weeks after baseline (R2). Serum samples were taken at each time point and cytokines concentrations determined by ELISA. RESULTS: Following treatment, statistically significant reductions were noted in clinical parameters. However, IL-17A and IL-17E concentrations were significantly greater than baseline values before- and after-adjusting for smoking. The IL-17A:IL-17E ratio was lower at R1 and R2. Serum IL-6 and TNF levels were significantly lower at R1 only. Also exclusively at R1, serum IL-17A and IL-17E correlated positively with clinical parameters, while the IL-17A:IL-17E ratio correlated negatively with probing pocket depth and clinical attachment. CONCLUSION: Increased serum IL-17E and a reduced IL-17A:IL-17E ratio may be indicative and/or a consequence of periodontal therapy. Therefore, the role of IL-17E in periodontal disease progression and the healing process is worthy of further investigation. CLINICAL RELEVANCE: IL-17E may be a valuable biomarker to monitor the healing process following periodontal treatment as increased IL-17E levels and a reduced IL-17A:IL-17E ratio could reflect clinical improvements post-therapy. Therefore, monitoring serum IL-17E might be useful to identify individuals who require additional periodontal treatment.
Authors: V R Santos; F V Ribeiro; J A Lima; T S Miranda; M Feres; M F Bastos; P M Duarte Journal: J Periodontal Res Date: 2011-08-02 Impact factor: 4.419
Authors: Mehmet A Eskan; Ravi Jotwani; Toshiharu Abe; Jindrich Chmelar; Jong-Hyung Lim; Shuang Liang; Paul A Ciero; Jennifer L Krauss; Fenge Li; Martina Rauner; Lorenz C Hofbauer; Eun Young Choi; Kyoung-Jin Chung; Ahmed Hashim; Michael A Curtis; Triantafyllos Chavakis; George Hajishengallis Journal: Nat Immunol Date: 2012-03-25 Impact factor: 25.606