PURPOSE: Acetylcarnitine formation is suggested to be crucial in sustaining metabolic flexibility and glucose homeostasis. Recently, we introduced a method to detect acetylcarnitine in vivo with long TE 1 H-MRS. Differences in T1 relaxation time between lipids and acetylcarnitine can be exploited for additional lipid suppression in subjects with high myocellular lipid levels. METHODS: Acquisition of spectra with an inversion recovery sequence was alternated with standard signal acquisition to suppress short T1 metabolite signals. A proof of principle experiment was conducted in a lean subject and the new approach was subsequently tested in four overweight/obese subjects. RESULTS: Using the new T1 editing approach, lipid signals in spectra of skeletal muscle can be (additionally) suppressed by a factor of 10 using a TI of 900 ms. Combination of the long TE protocol with the T1 editing resulted in a well-resolved acetylcarnitine peak in the obese subjects. CONCLUSION: The T1 editing approach suppresses short T1 metabolites and offers a new contrast in 1 H-MRS. The approach should be used in combination with a long TE in subjects with high lipid contamination for accurate quantification of the acetylcarnitine concentration. Magn Reson Med 77:505-510, 2017.
PURPOSE:Acetylcarnitine formation is suggested to be crucial in sustaining metabolic flexibility and glucose homeostasis. Recently, we introduced a method to detect acetylcarnitine in vivo with long TE 1 H-MRS. Differences in T1 relaxation time between lipids and acetylcarnitine can be exploited for additional lipid suppression in subjects with high myocellular lipid levels. METHODS: Acquisition of spectra with an inversion recovery sequence was alternated with standard signal acquisition to suppress short T1 metabolite signals. A proof of principle experiment was conducted in a lean subject and the new approach was subsequently tested in four overweight/obese subjects. RESULTS: Using the new T1 editing approach, lipid signals in spectra of skeletal muscle can be (additionally) suppressed by a factor of 10 using a TI of 900 ms. Combination of the long TE protocol with the T1 editing resulted in a well-resolved acetylcarnitine peak in the obese subjects. CONCLUSION: The T1 editing approach suppresses short T1 metabolites and offers a new contrast in 1 H-MRS. The approach should be used in combination with a long TE in subjects with high lipid contamination for accurate quantification of the acetylcarnitine concentration. Magn Reson Med 77:505-510, 2017.
Authors: Radka Klepochová; Ladislav Valkovič; Martin Gajdošík; Thomas Hochwartner; Harald Tschan; Michael Krebs; Siegfried Trattnig; Martin Krššák Journal: Invest Radiol Date: 2017-07 Impact factor: 6.016
Authors: Carlijn M E Remie; Kay H M Roumans; Michiel P B Moonen; Niels J Connell; Bas Havekes; Julian Mevenkamp; Lucas Lindeboom; Vera H W de Wit; Tineke van de Weijer; Suzanne A B M Aarts; Esther Lutgens; Bauke V Schomakers; Hyung L Elfrink; Rubén Zapata-Pérez; Riekelt H Houtkooper; Johan Auwerx; Joris Hoeks; Vera B Schrauwen-Hinderling; Esther Phielix; Patrick Schrauwen Journal: Am J Clin Nutr Date: 2020-08-01 Impact factor: 7.045
Authors: Martin Krššák; Lucas Lindeboom; Vera Schrauwen-Hinderling; Lidia S Szczepaniak; Wim Derave; Jesper Lundbom; Douglas Befroy; Fritz Schick; Jürgen Machann; Roland Kreis; Chris Boesch Journal: NMR Biomed Date: 2020-02-05 Impact factor: 4.044