Xiaoyan Pan1, Xiyan Wang1, Zhengchao Wang2, Xuenan Wang3, Zhaohua Dou1, Zhixin Li1. 1. Department of Histology and Embryology, Jilin Medical University Jilin 132013, China. 2. Provincial Key Laboratory for Developmental Biology and Neuroscience, College of Life Science, Fujian Normal University Fuzhou 350108, Fujian, China. 3. Reproductive Medicine Center, Affiliated Hospital of Jining Medical College Jining 272029, Shandong, China.
Abstract
OBJECTIVE: This study is to investigate effects of bisphenol A (BPA) on the blastocyst implantation in endometrium. METHODS: Pregnant mice were orally administered with BPA. Implantation sites were examined, and serum estrogen level was assayed with ELISA. Protein expression levels were detected with immunohistochemistry and immunofluorescence staining. RESULTS: High doses (400 and 600 mg/kg/day) of BPA remarkably reduced the implantation sites in the pregnant mice. No significant differences were observed in the serum estrogen level across the groups. Moreover, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression level of endometrial estrogen receptor α (ERα) in the pregnant mice. In addition, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression levels of integrin β3 and trophinin in the endometrium and blastocysts. CONCLUSION: BPA declines ERα expression in endometrium, and inhibits adhesion protein expression in endometrium and blastocysts, causing the adhesion failure of blastocyst implantation.
OBJECTIVE: This study is to investigate effects of bisphenol A (BPA) on the blastocyst implantation in endometrium. METHODS: Pregnant mice were orally administered with BPA. Implantation sites were examined, and serum estrogen level was assayed with ELISA. Protein expression levels were detected with immunohistochemistry and immunofluorescence staining. RESULTS: High doses (400 and 600 mg/kg/day) of BPA remarkably reduced the implantation sites in the pregnant mice. No significant differences were observed in the serum estrogen level across the groups. Moreover, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression level of endometrial estrogen receptor α (ERα) in the pregnant mice. In addition, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression levels of integrin β3 and trophinin in the endometrium and blastocysts. CONCLUSION:BPA declines ERα expression in endometrium, and inhibits adhesion protein expression in endometrium and blastocysts, causing the adhesion failure of blastocyst implantation.
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