BACKGROUND: MicroRNAs (miRNAs) play key roles in cardiac development, and the expression of miRNAs is altered in the diseased heart. The aim of this study was to explore the value of circulating microRNA-122-5p (miR-122-5p) as a potential biomarker for acute myocardial infarction (AMI). METHODS: Plasma samples from 50 patients with AMI and 39 healthy adults (non-AMI controls) were collected. The abundance of circulating miR-122-5p was measured using quantitative real-time PCR (qRT-PCR). The cTnI concentrations of these samples were analyzed by ELISA. RESULTS: Our findings revealed that circulating miR-122-5p expression were increased in AMI patients at 4 h, 8 h, 12 h, and 24 h by contrast to those non-AMI controls and displayed similar trends to that of cTnI concentrations in AMI patients. Further study showed that there is a high correlation between circulating miR-122-5p and cTnI concentrations. At last, the receiver operating characteristic (ROC) curve was performed and showed that circulating miR-122-5p had considerable diagnostic accuracy for AMI with an area under curve (AUC) of 0.855. CONCLUSIONS: Our results implied that circulating miR-122-5p could be a potential biomarker for AMI.
BACKGROUND: MicroRNAs (miRNAs) play key roles in cardiac development, and the expression of miRNAs is altered in the diseased heart. The aim of this study was to explore the value of circulating microRNA-122-5p (miR-122-5p) as a potential biomarker for acute myocardial infarction (AMI). METHODS: Plasma samples from 50 patients with AMI and 39 healthy adults (non-AMI controls) were collected. The abundance of circulating miR-122-5p was measured using quantitative real-time PCR (qRT-PCR). The cTnI concentrations of these samples were analyzed by ELISA. RESULTS: Our findings revealed that circulating miR-122-5p expression were increased in AMI patients at 4 h, 8 h, 12 h, and 24 h by contrast to those non-AMI controls and displayed similar trends to that of cTnI concentrations in AMI patients. Further study showed that there is a high correlation between circulating miR-122-5p and cTnI concentrations. At last, the receiver operating characteristic (ROC) curve was performed and showed that circulating miR-122-5p had considerable diagnostic accuracy for AMI with an area under curve (AUC) of 0.855. CONCLUSIONS: Our results implied that circulating miR-122-5p could be a potential biomarker for AMI.
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Authors: Till Keller; Tanja Zeller; Dirk Peetz; Stergios Tzikas; Alexander Roth; Ewa Czyz; Christoph Bickel; Stephan Baldus; Ascan Warnholtz; Meike Fröhlich; Christoph R Sinning; Medea S Eleftheriadis; Philipp S Wild; Renate B Schnabel; Edith Lubos; Nicole Jachmann; Sabine Genth-Zotz; Felix Post; Viviane Nicaud; Laurence Tiret; Karl J Lackner; Thomas F Münzel; Stefan Blankenberg Journal: N Engl J Med Date: 2009-08-27 Impact factor: 91.245
Authors: Sven W Meckelmann; Jade I Hawksworth; Daniel White; Robert Andrews; Patricia Rodrigues; Anne O'Connor; Jorge Alvarez-Jarreta; Victoria J Tyrrell; Christine Hinz; You Zhou; Julie Williams; Maceler Aldrovandi; William J Watkins; Adam J Engler; Valentina Lo Sardo; David A Slatter; Stuart M Allen; Jay Acharya; Jacquie Mitchell; Jackie Cooper; Junken Aoki; Kuniyuki Kano; Steve E Humphries; Valerie B O'Donnell Journal: Circ Genom Precis Med Date: 2020-05-12