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Literature DB >> 26883708

Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems.

Carlo Bottoni¹, Mariagrazia Perilli¹, Francesca Marcoccia¹, Alessandra Piccirilli¹, Cristina Pellegrini¹, Martina Colapietro¹, Alessia Sabatini¹, Giuseppe Celenza¹, Frédéric Kerff², Gianfranco Amicosante¹, Moreno Galleni², Paola Sandra Mercuri³.

Abstract

Site-directed mutagenesis of CphA indicated that prolines in the P158-P172 loop are essential for the stability and the catalytic activity of subclass B2 metallo-β-lactamases against carbapenems. The sequential substitution of proline led to a decrease of the catalytic efficiency of the variant compared to the wild-type (WT) enzyme but also to a higher affinity for the binding of the second zinc ion.

Entities: Chemical

Mesh：

Substances：

Year: 2016 PMID： 26883708 PMCID： PMC4862501 DOI： 10.1128/AAC.01703-15

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

7 in total

1. Update of the standard numbering scheme for class B beta-lactamases.

Authors: Gianpiero Garau; Isabel García-Sáez; Carine Bebrone; Christine Anne; Paola Mercuri; Moreno Galleni; Jean-Marie Frère; Otto Dideberg
Journal: Antimicrob Agents Chemother Date: 2004-07 Impact factor: 5.191

2. Biochemical characterization of Sfh-I, a subclass B2 metallo-beta-lactamase from Serratia fonticola UTAD54.

Authors: Fátima Fonseca; Christopher J Arthur; Elizabeth H C Bromley; Bart Samyn; Pablo Moerman; Maria José Saavedra; António Correia; James Spencer
Journal: Antimicrob Agents Chemother Date: 2011-08-29 Impact factor: 5.191

3. Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability.

Authors: M Hernandez Valladares; A Felici; G Weber; H W Adolph; M Zeppezauer; G M Rossolini; G Amicosante; J M Frère; M Galleni
Journal: Biochemistry Date: 1997-09-23 Impact factor: 3.162

4. Crystal structure of Serratia fonticola Sfh-I: activation of the nucleophile in mono-zinc metallo-β-lactamases.

Authors: Fátima Fonseca; Elizabeth H C Bromley; Maria José Saavedra; António Correia; James Spencer
Journal: J Mol Biol Date: 2011-07-06 Impact factor: 5.469

5. High specificity of cphA-encoded metallo-beta-lactamase from Aeromonas hydrophila AE036 for carbapenems and its contribution to beta-lactam resistance.

Authors: B Segatore; O Massidda; G Satta; D Setacci; G Amicosante
Journal: Antimicrob Agents Chemother Date: 1993-06 Impact factor: 5.191

6. The structure of the dizinc subclass B2 metallo-beta-lactamase CphA reveals that the second inhibitory zinc ion binds in the histidine site.

Authors: Carine Bebrone; Heinrich Delbrück; Michaël B Kupper; Philipp Schlömer; Charlotte Willmann; Jean-Marie Frère; Rainer Fischer; Moreno Galleni; Kurt M V Hoffmann
Journal: Antimicrob Agents Chemother Date: 2009-08-03 Impact factor: 5.191

7. The 3-D structure of a zinc metallo-beta-lactamase from Bacillus cereus reveals a new type of protein fold.

Authors: A Carfi; S Pares; E Duée; M Galleni; C Duez; J M Frère; O Dideberg
Journal: EMBO J Date: 1995-10-16 Impact factor: 11.598

7 in total

3 in total